Sclerotinia sclerotiorum is a devastating plant pathogen that causes substantial losses in various agricultural crops. Although plants have developed some well-known defense mechanisms against invasive fungi, much remains to be learned about responses fungal pathogens. In this study, we investigated how S. infection affects primary and secondary metabolism the model Arabidopsis thaliana. Our re...

Biotrophic pathogens are believed to strategically manipulate sugar transport in host cells enhance their access carbohydrates. However, mechanisms of translocation from biotrophic fungi such as powdery mildew across the plant–haustorium interface remain poorly understood. To investigate this question, systematic subcellular localisation analysis was performed for all 14 members monosaccharide ...

Arabidopsis thaliana is the first plant for which the complete genome has been sequenced and published. Annotation of complex eukaryotic genomes requires more than the assignment of genetic elements to the sequence. Besides completing the list of genes, we need to discover their cellular roles, their regulation and their interactions in order to understand the workings of the whole plant. The M...

The glyoxalase pathway, which consists of the two enzymes, GLYOXALASE 1 (GLX 1) (E.C.: 4.4.1.5) and 2 (E.C.3.1.2.6), has a vital role in chemical detoxification. In Arabidopsis thaliana there are at least four different isoforms of glyoxalase 2, two of which, GLX2-1 and GLX2-4 have not been characterized in detail. Here, the functional role of Arabidopsis thaliana GLX2-1 is investigated. Glx2-1...

A direct interaction of the Arabidopsis thaliana immunophilin ROF1 with phosphatidylinositol-3-phosphate and phosphatidylinositol-3,5-bisphosphate was identified using a phosphatidylinositol-phosphate affinity chromatography of cell suspension extracts, combined with a mass spectrometry (nano LC ESI-MS/MS) analysis. The first FK506 binding domain was shown sufficient to bind to both phosphatidy...

Title of Thesis: TIME SERIES TRANSCRIPTIONAL PROFILING ANALYSIS OF THE Arabidopsis thaliana USING FULL GENOME DNA MICROARRAY AND METABOLIC INFORMATION. Bhaskar Dutta, Master of Science, 2004 Thesis Directed By: Dr. Maria Klapa, Assistant Professor Department of Chemical Engineering Dr. John Quackenbush, Investigator The Institute for Genomic Research With the advent of the DNA microarray techno...

Basic helix-loop-helix (bHLH) transcription factors represent a family of proteins that contain a bHLH domain, a motif involved in binding DNA. Recently, two groups independently analyzed the BHLH gene family of Arabidopsis thaliana (Heim et al., 2003; Toledo-Ortiz et al., 2003). These analyses revealed that this family is one of the largest transcription factor gene families in Arabidopsis tha...

Evolution of chromosome complements can be resolved by genome sequencing, comparative genetic mapping, and comparative chromosome painting. Previously, comparison of genetic maps and gene-based phylogenies suggested that the karyotypes of Arabidopsis thaliana (n = 5) and of related species with six or seven chromosome pairs were derived from an ancestral karyotype with eight chromosome pairs. T...

Abstract Plants synthesize specialized metabolites to facilitate environmental and ecological interactions. During evolution, plants diversified in their potential these metabolites. Quantitative differences metabolite levels of natural Arabidopsis (Arabidopsis thaliana) accessions can be employed unravel the genetic basis for metabolic traits using genome-wide association studies (GWAS). Here,...