Production of alkaline protease from Bacillus licheniformis (NCIM 2044) was studied and characterized. Of different cheap source media chosen, maximum production of 172.425 U/ml was obtained for 1% wheat bran containing media. The enzyme assay at various optimum pH and temperatures were also determined.

The considerations are discussed that make continuous monitoring of reaction-progress curves superior to fixed-incubation methods in the determination of enzyme activity. Provided that they are used with caution and their limitations are appreciated, fixedincubation methods continue to fulfill a useful, though diminishing, role in clinical enzymology because of their adaptability to existing pa...

Exoribonucleases-among the other RNases-play a crucial role in the regulation of different aspects of RNA metabolism in the eukaryotic cell. To fully understand the exact mechanism of activity exhibited by such enzymes, it is crucial to determine their detailed biochemical properties, notably their substrate specificity and optimal conditions for enzymatic action. One of the most significant fe...