نتایج جستجو برای: asymmetric pcr
تعداد نتایج: 240327 فیلتر نتایج به سال:
mechanism design theory and its role in credence goods market; emphasizing on an islamic perspective
credence goods market is one of the markets that the asymmetric information has transformed it to a missing market. those goods and services that the applicant cannot determine the exact amount of their expenses due to the asymmetric information are called credence goods. from the economists' perspective, transaction in a market where participants are ignorant about each other is a game wi...
It is well known that some genetic mutations have a critical impact on protein structure and function. To study the effects of these mutations, site-directed mutagenesis allows the introduction of a defined substitution of specific residues. For the past few years, numerous PCR-based methods have been employed for achieving site-directed mutagenesis. Among these methods, megaprimer PCR has been...
Fast, portable and sensitive analysis of E. coli is becoming an important challenge in many critical fields (e.g., food safety, environmental monitoring and clinical diagnosis). Thus, electrochemical biosensing of PCR amplicons from the bacterial genome has attracted reasonable research attention. In this work, we utilized a 3D DNA tetrahedral probe to establish a "sandwich-type" electrochemica...
Traditional asymmetric PCR uses conventional PCR primers at unequal concentrations to generate single-stranded DNA. This method, however, is difficult to optimize, often inefficient, and tends to promote nonspecific amplification. An alternative approach, Linear-After-The-Exponential (LATE)-PCR, solves these problems by using primer pairs deliberately designed for use at unequal concentrations....
Microfabricated silicon/glass-based devices with functionalities of simultaneous polymerase chain reaction (PCR) target amplification and sequence-specific electrochemical (EC) detection have been successfully developed. The microchip-based device has a reaction chamber (volume of 8 microl) formed in a silicon substrate sealed by bonding to a glass substrate. Electrode materials such as gold an...
During asymmetric division, a cell polarizes and differentially distributes components to its opposite ends. The subsequent division differentially segregates the two component pools to the daughters, which thereby inherit different developmental directives. In Drosophila sensory organ precursor cells, the localization of Numb protein to the cell's anterior cortex is a key patterning event and ...
We intend to show that transient chaos is a very appealing, but still not widely appreciated, subfield of nonlinear dynamics. Besides flashing its basic properties and giving a brief overview of the many applications, a few recent transient-chaos-related subjects are introduced in some detail. These include the dynamics of decision making, dispersion, and sedimentation of volcanic ash, doubly t...
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