BACKGROUND In countries with high tuberculosis (TB) burden, there is urgent need for rapid, large-scale screening to detect smear-positive patients. We developed a computer-aided whole smear screening system that focuses in real-time, captures images and provides diagnostic grading, for both bright-field and fluorescence microscopy for detection of acid-fast-bacilli (AFB) from respiratory speci...

Various synthetic dyes are artificially added to herbal medicines for the purpose of visual attraction. In order monitor illegal usage in medication, a rapid and straightforward analysis method determine is required. The study aimed develop validate high-performance liquid chromatography (HPLC) ten Hawthorn fruit, Cornus Schisandra fruit. Ten such as Tartrazine, Sunset yellow, Metanil Auramine ...

BACKGROUND AND PURPOSE Cryptosporidium is a protozoan parasite that reproduces within the epithelial cells of several organs of vertebrate hosts. The manifestation of the disease is either self-limiting acute diarrhea in immunocompetent patients, or fatal chronic diarrhea in immunocompromised patients. Common clinical symptoms include watery diarrhea, abdominal pain, and weight loss. METHODS ...

Cryptosporidium spp is now widely accepted as a cause of gastroenteritis. Various methods have been applied to detect oocysts in faeces, but the difficulties of discriminating between non-cryptosporidial bodies, acid fast bodies like cryptosporidia, and cryptosporidia remain. A simple examination in two stages, suitable for routine use is described, using auramine phenol and carbol fuchsine for...

OBJECTIVE To use an automated method to detect tubercle bacilli in sputum specimens. STUDY DESIGN Using fluorescence microscopy, tubercle bacilli were identified on auramine-stained sputum specimens. Images were then captured with a digital camera and enhanced through imaging processing techniques. The bacilli were recognized using neural network classifiers. RESULTS This preliminary invest...

BACKGROUND To quantify the likely impact of recent WHO policy recommendations regarding smear microscopy and the introduction of appropriate low-cost fluorescence microscopy on a) case detection and b) laboratory workload. METHODOLOGY/PRINCIPAL FINDINGS An audit of the laboratory register in an urban hospital, Lilongwe, Malawi, and the application of a simple modelling framework. The adoption...

The fluorescence of tubercle bacilli stained with auramine and rhodamine does not require ultra-violet light. It can be caused by blue light up to 406mp. A’copper sdphate ammonia liquid filter, suitably diluted, will transmit this waveband. A gelatin screen filter, absorbing all light below 510mp. is used in the eyepiece. Normal, high intensity projection Alament lamps, combined with lamp conde...

We report two immigrants with tuberculosis of the skull base and a review of the literature. A Somalian man presented with bilateral otitis media, hearing loss, and facial and abducens palsy. Imaging showed involvement of both mastoid and petrous bones, extending via the skull base to the nasopharynx, suggesting tuberculosis which was confirmed by characteristic histology and positive auramine ...