We examined the suitability of high-performance immunoaffinity chromatography (HPIAC) for removing beta 2-microglobulin (beta 2-MG) from plasma of patients (n = 26) undergoing long-term hemodialysis. We used immobilized antibody to beta 2-MG to prepare an immunoaffinity column with a 0.5-mL bed volume. Plasma samples of 0.5 mL were chromatographed at a flow rate of 0.5 mL/min and pressures < 7 ...

The results of scintiphotography with gallium-67 (67Ga), renography with technetium-99m diethylene triamine penta-acetic acid, immunofluorescence of antibody coated bacteria, and determination of renal beta 2 microglobulin excretion were compared in 19 patients with upper and 15 patients with lower urinary tract infection. All patients with acute pyelonephritis showed an appreciable unilateral ...

Expression of MHC class I antigens requires the intracellular assembly of HLA-A,B,C H chains and beta 2-microglobulin (beta 2m). We have investigated the expression of free H chain, beta 2m, and their mRNAs in tissue sections of colon carcinomas that were defective for the native two-chain molecule. In all of these tumors, beta 2m protein and mRNA were found to be completely absent from the neo...

serum ige and beta 2 microglobulin levels were determined in 31 patients suffering from acute myocardial infarction and 30 patients with other forms of ischemic heart disease. the levels of these parameters were studied on the first, third and seventh day after the onset of disease. the immunological method used for the determinations was elisa patients with myocardial infarction showed an elev...

PURPOSE Urinary angiotensinogen has been reported as a marker for the activation of intrarenal renin-angiotensin system (RAS) in various kidney diseases. To investigate the importance of urinary angiotensinogen in diabetic nephropathy, we compared the urinary levels of angiotensinogen, albumin, and α1-microglobulin. MATERIALS AND METHODS Japanese patients with type 2 diabetes at various stage...

Our aim was to investigate whether serum neopterin and beta 2-microglobulin have any value in the distinction between Pneumocystis carinii pneumonia (PCP) and pneumonia due to extracellular bacteria. Also, to study whether neopterin and beta 2-microglobulin would correlate with the clinical course of lung infections in human immunodeficiency virus (HIV)-positive and HIV-negative patients. Thirt...

Thirteen newborn infants, 8 term and 5 preterm (gestational age 31 to 36 weeks), were treated for between 3 and 7 days with gentamicin and ampicillin or cloxacillin because of suspected bacterial infection. The dosage of gentamicin was carefully monitored by serum concentration assays. Urinary alanine aminopeptidase, urinary beta 2-microglobulin, serum urea, and serum beta 2-microglobulin were ...

beta 2 Microglobulin concentration in tear fluid was measured in 35 patients with primary Sjögren's syndrome (SS), in 28 normal control subjects matched for age and sex, and in 18 patients with arthralgias or myalgias and sicca complaints, in whom the diagnosis primary SS had been excluded. Increased beta 2 microglobulin concentrations were found in the patients with SS, but no correlation was ...