A non-radioactive in situ hybridization method is described for the localization of transcription units of defined genes to lateral loops of Xenopus laevis lampbrush chromosomes. Two Xenopus cDNA probes were used encoding the nucleolar protein NO38/B23 and cytokeratin 1(8). Both proteins are known to be synthesized in Xenopus oocytes, and Northern blot analysis revealed the presence of the corr...

Previous studies have shown that pretreatment with sub-lethal kanamycin to E. coli B23 cells provided protection against subsequent exposure to lethal amounts of the same antibiotic. It has also been shown that exposure to sub-inhibitory levels of certain antibiotic can induce cross resistance by promoting the development of low-level resistance to other functional and structural unrelated anti...

1 Immunology Unit, Pathology & Clinical Laboratory Medicine, King Fahad Medical City, Box 59046, Riyadh 11525, Saudi Arabia 2Laboratory of Immunoendocrinology, Institute of Pathology CHU-B23, University of Liege Center of Immunology (CIL), 4000 Liege-Sart Tilman, Belgium 3Department of Pathology and Immunology, Diabetes Institute, Rangos Research Center, University of Pittsburgh School of Medic...

abstract: this paper discusses two topics. at first, it uses continuous wavelet (morlet) transform, coherency, and phase angle analysis to study the effect of mean volatility in opec crude oil prices, wti crude oil prices, brent crude oil prices and iran’s crude oil prices on the iran’s reer. to this end, the monthly data of the variables for years 2003 to 2012 is used. coherency analysis revea...

Nuclei assembled in Xenopus egg extract contain numerous spherical aggregations or nuclear bodies. Previously we have shown that they closely resemble prenucleolar bodies (PNBs), both at the compositional and ultrastructural level. Subsequently, coilin was also identified and for this reason they were called coiled bodies. Here we present morphological and immunocytochemical evidence that the i...

We report the molecular characterization of a novel nucleolar protein, Nop52, and its subcellular distribution during the cell cycle and nucleologenesis. This protein was originally identified with human autoantibodies which were subsequently used to clone its corresponding cDNA. Transfection experiments in mammalian cells have confirmed that this cDNA encodes a nucleolar protein that accumulat...

The nucleolus is the site of ribosome biosynthesis, but is now known to have other functions as well. In the present study we have investigated how the distribution of signal recognition particle (SRP) RNA within the nucleolus relates to the known sites of ribosomal RNA synthesis, processing, and nascent ribosome assembly (i.e., the fibrillar centers, the dense fibrillar component (DFC), and th...

Astyanax douradilho, new species, is described from the rio Tramandaí system, coastal drainage of Rio Grande do Sul State, Brazil. The new species is distinguished from its congeners by possessing 37-39 perforated scales along the lateral line, 3-5 maxillary tricuspid teeth, 22-24 branched anal-fin rays, absence of a conspicuous dark stripe from humeral region to caudal peduncle, two vertically...