Neutralizing activity against T2 bacteriophage appeared in cultures of lymph node cells from normal rats in response to their in vitro stimulation with a cell-free filtrate derived from homogenized rat macrophages which had been incubated with T2 bacteriophage. This activity was specifically directed against T2 bacteriophage. It resided in a fraction of the culture fluid which had the salting-o...

This study aimed to inhibit Escherichia coli (E. coli) O157:H7 artificially contaminated in fresh meat using bacteriophage. Among 14 bacteriophages, the highly lytic bacteriophage BPECO19 strain was selected to inhibit E. coli O157:H7 in artificially contaminated meat samples. Bacteriophage BPECO19 significantly reduced E. coli O157:H7 bacterial load in vitro in a multiplicity of infection (MOI...

Lactococcus lactis subsp. lactis biovar. diacetylactis DPC721 is a spontaneous bacteriophage insensitive mutant of strain DPC220, isolated after challenge with an industrial bacteriophage, phi D1. Plasmid analysis demonstrated that the bacteriophage insensitivity was associated with the absence of two native DPC220 plasmids (pAH82 and pAH33), and the presence of a novel plasmid (pAH90) in DPC72...

Since the discovery of bacteriophage in the early 1900s, there have been numerous attempts to exploit their innate ability to kill bacteria. The purpose of this report is to review current findings and new developments in phage therapy with an emphasis on bacterial diseases of marine organisms, humans, and plants. The body of evidence includes data from studies investigating bacteriophage in ma...

Pseudomonas ΦKZ-like bacteriophages encode a group of related tubulin/FtsZ-like proteins believed to be essential for the correct centring of replicated bacteriophage virions within the bacterial host. In this study, we present crystal structures of the tubulin/FtsZ-like protein TubZ from Pseudomonas bacteriophage ΦKZ in both the monomeric and protofilament states, revealing that ΦKZ TubZ under...

cultures of lactic streptococci. J. Dairy Research, 5, 197-207. WHITEHEAD, H. R., AND COX, G. A. 1936 Bacteriophage phenomena in cultures of lactic streptococci. J. Dairy Research, 7, 55-62. WHITEHEAD, H. R., AND HUNTER, G. J. E. 1937 Observations on the activity of bacteriophage in the group of lactic streptococci. J. Path. Bacteriol., 44, 337-347. WHITEHEAD, H. R., AND HUNTER, G. J. E. 1947 B...

The inactivation of staphylococcus bacteriophage by an artificially produced anti-phagic serum was first reported by Gratia and Jaumain", who also found that normal rabbit serum inhibits staphy phage,-in some instances to a titer. as high as 1: 500. More recently it has been observed (Rosenthal29, Raiga24 25, Biglieri and Fischer4, Applebaum and MacNeal2, Colvin8, and Evans"0) that normal human...

We have developed a rapid and reliable bacteriophage quantification method based on measurement of phage single-stranded DNA (ssDNA) using switchable lanthanide chelate complementation probes. One oligonucleotide probe contains a non-fluorescent lanthanide ion carrier chelate and another probe is labeled with a light absorbing antenna ligand. Hybridization of the non-fluorescent complementation...

4. SEALING OF DNA FRAGMENTS 219 4.1. E. coli DNA ligase 4.2. Bacteriophage T 4 gene 30 protein 4.3. Mammalian DNA ligases I and II C O N F O R M A T I O N A L CHANGES OF DNA DURING REPLICATION 220 5.1. E. coli HDP 5.2. Bacteriophage-coded HDPs — T 4 gene 32 protein —T7 HDP —fd gene V protein 5.3. Eukaryotic HDPs 5.4. Bacterial DNA topoisomerases —Swivelases —DNA gyrase 5.5. Bacteriophage-coded ...

Controlling the assembly of basic structural building blocks in a systematic and orderly fashion is an emerging issue in various areas of science and engineering such as physics, chemistry, material science, biological engineering, and electrical engineering. The self-assembly technique, among many other kinds of ordering techniques, has several unique advantages and the M13 bacteriophage can b...