Production of baicalein, baicalin and wogonin by liquid culture of Scutellaria baicalensis cells derived from the plant root was studied. The maximum production obtained were 119 mg/L of baicalein at two week, 1372 mg/L of baicalin at eight week, and 14 mg/L of wogonin at two week. In addition, the production of baicalin was drastically increased to 1000 mg/L level at 3-week culture, and the ex...

Baicalin, a type of flavonoid extracted from the dried root of Scutellaria baicalensis georgi, has been shown to effectively inhibit cell apoptosis. Therefore, we assumed that baicalin would suppress co-listin sulfate-induced neuronal apoptosis. PC12 cells exposed to colistin sulfate (62.5-500 μg/mL) for 24 hours resulted in PC12 cell apoptosis. In addition, caspase-3 activity, lactate dehydrog...

the objective of this study was to observe the apoptosis-inducing effect and mechanism of baicalin on human cervical cancer hela cells. the inhibitory effect of baicalin on the growth of hela cells was measured by mtt assay, and cell proliferation and migration was analyzed by cell scratch assay. morphological changes of apoptotic cells were viewed by the light microscope and electron microscop...

The aim of this study was to optimize the preparation process of baicalin-β-cyclodextrin inclusion compound (baicalin-β-CD) by adopting Response Surface Methodology (RSM). On the basis of the single-factor experiment, the conditions of the inclusion process were optimized by adopting RSM with the factors to be investigated (i.e. the proportion of β-CD and baicalin, inclusion time and inclusion ...

Objectives: Baicalin is the main bioactive flavonoid constituent isolated from Scutellaria baicalensis Georgi. The mechanisms of protection of liver remain unclear. In this study, 1H NMR-based metabonomics approach has been used to investigate the alleviation effect of Baicalin. Method:1H NMR metabolomics analyses of urine and serum from rats, was performed to illuminate the alleviation effect ...

Baicalin is a widely used compound in Chinese traditional medicine and exhibits many pharmacological activities. Under simulated physiological conditions, the interaction between baicalin and human serum albumin(HSA), had been investigated by fluorescence spectroscopic, ultraviolet spectrum(UV), Fourier transform infrared(FT-IR), circular dichroism(CD) and molecular modeling. The results indica...

To explore the effect of baicalin on cognitive function and nerves after splenectomy in aged rats is main objective study. Aged were randomly divided into a sham operation group, model group (to construct surgical model) treatment (baicalin was given constructed). The pathological conditions like interleukin-6, tumor necrosis factor alpha, interleukin-1 beta content; brain detection by terminal...

OBJECTIVE To establish a high-performance liquid chromatography (HPLC) method for the determination of baicalin in Xiaoyanling Decoction. METHODS HPLC method was used for determination. A Kromosil C18 column was used. The mobile phase consisted of MeOH-acid water (H(2)O:H(3)PO(4)=53:0.2) 40:60, and the detective wavelength was 277 nm. RESULTS The content of baicalin in each Xiaoyanling Deco...

The aim of the present study was to determine the effect of baicalin on the expression of miR-497 and its target B-cell lymphoma-2 (Bcl-2) in the hippocampus of kainic acid (KA)-induced epileptic mice. To establish status epilepticus (SE), 0.1 µg/5 µl KA was injected into the lateral cerebral ventricle in mice, which then received an intraperitoneal injection of baicalin (100 mg/kg) after 1 and...

T(H)17 cells have been implicated in a growing list of inflammatory disorders. Antagonism of T(H)17 cells can be used for the treatment of inflammatory injury. Currently, very little is known about the natural compound controlling the differentiation of T(H)17 cells. Here, we showed that Baicalin, a compound isolated from a Chinese herb, inhibited T(H)17 cell differentiation both in vitro and i...