When a BCR on a mature B cell is engaged by its ligand, the cell becomes activated, and the Ab-mediated immune response can be triggered. The initiation of BCR signaling is orchestrated by kinases of the Src and Syk families. However, the proximal BCR-induced phosphorylation remains incompletely understood. According to a model of sequential activation of kinases, Syk acts downstream of Src fam...

In chronic myeloid leukemia (CML) two main types of messenger RNA (e14a2 and e13a2) can be produced by BCR-ABL1 gene rearrangement. Due to conflicting results, the clinical value of these transcripts remains controversial. The aim of this study was to identify associations of e14a2 and e13a2 transcripts with laboratory variables and also the response to treatment. This study included 203 adult ...

We explored the role of antigen valency in B cell receptor (BCR) activation and rearrangement of intracellular MHC class II compartments as factors that contribute to the efficacy of antigen presentation. Using primary B cells that express a hen egg lysozyme (HEL)-specific BCR, we found that oligomeric HEL more efficiently promoted both BCR activation and internalization than did monovalent HEL...

The assembly of immunoglobulin genes occurs in ordered waves during B cell development. The heavy chain (Igh) locus generally recombines first, and each allele has at most one chance to undergo a productive rearrangement. Subsequently, at the κ (Igk) locus, individual alleles can undergo sequential rounds of rearrangement , permitting different light-heavy chain combinations to be tested until ...

The objective of the present study was to investigate the reduction of mobility, availability and toxicity found in soil contaminated with lead (Pb) and cadmium (Cd) from Santo Amaro Municipality, Bahia, Brazil using two combined methods, commonly tested separately according to the literature: metal mobilization with phosphates and phytoextraction. The strategy applied was the treatment with tw...

introduction high concentrations of metals are usually encountered in surface soil and vegetation in areas affected by mining activity (liu et al., 2006). different distribution of elements in chemical fractions result in different bioavailability; therefore knowledge of the total content of an element in soil is not a sufficient criterion to estimate the environmental implications of trace met...

We conducted a modified Bureau Commun Reference (BCR) sequential extraction on a basaltic soil (phono-tephrite) from Mt. Meru in Northern Tanzania in order to determine the relative contribution of water soluble, carbonate and exchangeable, oxide and organic fractions to the bulk composition of the soil. Elemental compositions were determined by ICP-MS and corrected for loss on ignition. Relati...

The chemical speciation of heavy metals (arsenic, chromium, copper, lead, zinc and tin) in the sediments of former tin mining catchment Bestari Jaya, Peninsular Malaysia was determined by using the latest version of the Community Bureau of Reference, usually called the BCR 3-step sequential extraction procedure. Furthermore, a fourth step was introduced for digestion and analysis of the residue...

As waste management regulations become more stringent, yet demand for resources continues to increase, there is a pressing need for innovative management techniques and more sophisticated supporting analysis techniques. Sequential extraction (SE) analysis, a technique previously applied to soils and sediments, offers the potential to gain a better understanding of the composition of solid waste...