نتایج جستجو برای: benthosema pterotum luciferase

تعداد نتایج: 14338  

Journal: :Scientific reports 2015
Guo-Qing Wu Xiao Wang Hong-Ying Zhou Ke-Qun Chai Qian Xue Ai-Hong Zheng Xiu-Ming Zhu Jian-Yong Xiao Xu-Hua Ying Fu-Wei Wang Tao Rui Li-Yun Xu Yong-Kui Zhang Yi-Ji Liao Dan Xie Li-Qin Lu Dong-Sheng Huang

The dual-luciferase reporter assay is widely used for microRNA target identification and the functional validation of predicted targets. To determine whether curcumin regulates expression of the histone methyltransferase enhancer of zeste homolog 2 (EZH2) by targeting its 3'untranslated region (3'UTR), two luciferase reporter systems containing exactly the same sequence of the EZH2 3'UTR were u...

Journal: :Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 2005
Ibtisam Ghazawi Samuel J Cutler Pauline Low Albert S Mellick Stephen J Ralph

Luciferase reporter constructs are widely used for analysis of gene regulation when characterizing promoter and enhancer elements. We report that the recently developed codon-modified Renilla luciferase construct included as an internal standard for cotransfection must be used with great caution with respect to the amount of DNA transfected. Also, the dual-luciferase reporter vectors encoding P...

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