Investigations were carried out with maize (Zea mays L.) seedlings. The effect of chlorsulfuron (CHF) on the electrophoretic profiles of the total and thermostable proteins, polypeptides and some antioxidant enzymes was compared to the effect of abscisic acid (ABA), benzylaminopurine (BAP) and N1-(2-chloro-4-pyridyl)-N2-phenylurea (4PU-30) applied in concentrations that inhibit the growth of se...

A high frequency shoot organogenesis and plant establishment protocol has been developed for Gerbera jamesonii from ex vitro leaf derived callus. The optimal callus was developed on Murashige and Skoog (MS) basal medium supplemented with 0.4 mg L 6-benzylaminopurine (BAP), 4.0 mg L -naphthalene acetic acid (NAA) and 3% (w/v) sucrose. Two callus types differing in their structures and growth rat...

Orchids have a long period of production and need to be under the right conditions. Therefore, in vitro propagation is an alternative reduce time. This study aimed determine optimal conditions two species orchids, Cattleya maxima Lindl. Phalaenopsis amabilis (L.) Blume. For this goal, experiments were carried out: multiplication rooting, for both species. In experiment, treatments differentiate...

Cymbidium erythrostylum Rolfe is one of the most beautiful species genus cymbidium which used as a cut flower or indoor plant. However, it was registered rare even in its original habitat. This study carried out to develop protocol for vitro propagation C. erythrostilum. We protosomes obtained by asymbiotic germination seed on 1/2 Murashige and Skoog nutrient medium (MS) supplemented with 1 mg/...

BACKGROUND AND AIMS Dendrobium longicornu, commonly known as the 'Long-horned Dendrobium', is an endangered and medicinally important epiphytic orchid. Over-exploitation and habitat destruction seriously threaten this orchid in Northeast India. Our objective was to develop an efficient protocol for the mass propagation of D. longicornu using axillary bud segments. METHODOLOGY AND PRINCIPAL RE...

Purpose. To optimize the method of clonal micropropagation rocket (Eruca sativa Mill.). Methods. Seeds cultivars 'Znahar' and 'Lybid' were used for introduction into in vitro culture. Solutions sodium hypochlorite (35%), ethanol (70%) HgCl2 (0.2%) sterilization. A 5% solution chloramine served as a control. Sterile material was planted on liquid solid agar nutrient media according to Murashige-...

Micropropagation of Vaccinium spp. using Murashige Skoog (MS) and benzylaminopurine (BAP) has been successfully developed. This work aimed to determine the effect different types substrates auxins (naftalen acetic acid NAA, indol IAA y, butyric IBA) on ex vitro establishment rooting nodal segments V. corymbosum Var. Biloxi create a donor bank blueberry plants. The influence five three concentra...

In Indonesia, the agribusiness sector has begun to expand due increase in entrepreneurship of orchid plants. Several genera and species plants are promising be traded as ornamental plants, one which is moon (Phalaenopsis amabilis). However, limited number seeds or produced by conventional propagation methods takes a long time get new tillers. Therefore, tissue culture techniques can used grow r...

Abstract In eggplant microspore embryogenesis, embryos are produced and then transformed into undifferentiated calli, instead of developing as true embryos. This is the main current bottleneck that precludes this process from being efficient. work we aimed to shed light on factors involved in successful vitro development haploid by evaluating role growth regulators (GRs) process. We analyzed en...

In the present investigation an attempt has been made to enhance rosmarinic acid level in plants, grown in vitro, of 2 species of Mentha in presence of 2 precursors in the nutrient media during culture. For in vitro culture establishment and shoot bud multiplication, MS basal media were used supplemented with different concentrations and combinations of different growth regulator like NAA (alph...