Fixation of HeLa cells with a mixture of 100 mM glutaraldehyde, 2 mg/ml tannic acid and 0.5 mg/ml saponin allows the tannic acid to penetrate intact cells without disruption of membranes or extraction of the cytoplasmic matrix. After subsequent treatment with OsO4 cytoplasmic structures are stained so densely that fine details are visible even in very thin (dark gray) sections. Actin filaments ...

The intention of this study was to compare the different parts Tunisian Ferula communis via their fatty acids, essential oils and phenolic compounds. Results showed that lipid fraction fruits leaves characterized by predominance oleic acid. Erucic linoleic acids were most abundant in stems while palmitic flowers. F. defined four chemotypes, namely isoshyobunone/6-tert-butyl-4-methylcoumarin ste...

ÐFerron reagent and FTIR spectroscopy were used for the identi®cation and characterization of the aluminum species formed during dephosphorization of simulated wastewater with and without organic matter. Evidence from FTIR spectroscopy showed the formation of aluminum hydroxyphosphate, hydroxy-Al±tannate and aluminum complexes containing both phosphorus and tannic acid. The surface reactivity o...

Chitosan-based films are promising for consideration as packaging materials. In this study, we modified the chitosan by phenolic acid addition, such ferulic acid, caffeic tannic and gallic acid. The mechanical thermal properties were studied, water vapor permeability rate was determined. Moreover, antioxidant activity film color considered. results showed that acids effective cross-linkers chit...

Callus cultures were established in vitro from leaf explants of Malva neglecta and were sub-cultured several times until a rapid-growing cell line was obtained. The calluses were exposed to different doses of ultraviolet (UV) radiation as follows: 0, 432, 864, and 1296 J/m2 for UV B and 0, 612, 1284, and 1836 J/m2 for UV C, corresponding to 0, 30, 60, and 90 min radiations, in tandem. Exposure ...

Endoplasmic reticulum (ER) stress is an intriguing target with significant clinical importance in chemotherapy. Interference with ER functions can lead to the accumulation of unfolded proteins, as detected by transmembrane sensors that instigate the unfolded protein response (UPR). Therefore, controlling induced UPR via ER stress with natural compounds could be a novel therapeutic strategy for ...

Organic compounds are capable of repelling motile bacteria from marine surfaces. The most effective compounds were acrylamide and benzoic and tannic acids. These were active at concentrations that were not toxic to the bacteria. Repellents were incorporated in nontoxic paints and applied to metal panels. Treated panels immersed in seawater developed a bacterial film of only 10(6) bacteria per c...

Thermal denaturing of human hemoglobin (HHb) in the presence of tannic acid (TA) was studied by fluorescence and UV/vis spectroscopy in 30–66°C temperature range. To study more detailed structural changes in HHb molecule caused by temperature rise in the presence of TA, the excitation/emission fluorescence (3D spectra) matrix method has been used. It has been shown, that the increase of tempera...

The preservation for electron microscopy of saturated phospholipids in general, and phosphatidyl choline (PC)in particular, remains and unsolved problem since OsO(4) and glutaraldehyde are incapable of interacting with PC directly. However, by introducing tannic acid preceding osmication, we were able to demonstrate highly ordered, preserved lamellar structures in model experiments with saturat...