background: dna damage is one of the major consequences of radiation exposure onto the biological systems. a series of compounds including flavanoids were found to render dna protection against radiation damage. in this study we elucidated the potential of rutin and rutin hydrate to protect plasmid dna against damage induced by irradiation. materials and methods: dpph and hydroxyl radical scave...

Substances that may act as estrogens show a broad chemical structural diversity. To thoroughly address the question of possible adverse estrogenic effects, reliable methods are needed to detect and identify the chemicals of these diverse structural classes. We compared three assays--in vitro estrogen receptor competitive binding assays (ER binding assays), yeast-based reporter gene assays (yeas...

it has been investigated the in vitro antioxidant properties of ethanol extracts of the contained-allantoin plants in this study. contained-allantoined plant samples plantago lanceolata, plantago major, robinia pseudoacacia, platanus orientalis and aesculus hippocastanum were tested at different concentrations. the antioxidant activities of plant samples were analysed by 1,1- diphenyl-2-picrylh...

Abstract The coronavirus disease 2019 (COVID‐19) pandemic has devastated families and disrupted healthcare, economies societies across the globe. Molecular recognition agents that are specific for distinct viral proteins critical components rapid diagnostics targeted therapeutics. In this work, we demonstrate selection of novel DNA aptamers bind to SARS‐CoV‐2 spike glycoprotein with high specif...

Sigma-1 receptor (S1R) is an intracellular, multi-functional, ligand operated protein that also acts as a chaperone. It considered pluripotent drug target in several pathologies. The publication of agonist and antagonist bound structures has paved the way for receptor-based silico design. However, recent studies on this subject payed no attention to structural differences binding. In work, we h...

despite the availability of various alkaline phosphatase (alp) isoenzymes, the calf enzyme is being used in current enzyme assays as the detector enzyme. the glycosylation pattern of this enzyme makes it a suitable ligand for binding to wheat germ agglutinin lectin (wga). as a result of this property, the enzyme can not be used as a conjugate with this lectin, and the calf enzyme conjugates can...

For a long time, it has been assumed that the mode of action of Cry2A toxins was unique and different from that of other three-domain Cry toxins due to their apparent nonspecific and unsaturable binding to an unlimited number of receptors. However, based on the homology of the tertiary structure among three-domain Cry toxins, similar modes of action for all of them are expected. To confirm this...

The influenza A virus nucleoprotein (NP) has been examined with regard to its RNA-binding characteristics. NP, purified from virions and devoid of RNA, bound synthetic RNAs in vitro and interacted with the ribonucleotide homopolymers poly(A), poly(G), poly(U), and poly(C) in a salt-dependent manner, showing higher binding affinity for polypyrimidine homopolymers. To map the NP regions involved ...