A rapid, ultra high-performance liquid chromatographic (UHPLC) method has been developed and validated for simultaneous identification and analysis of the isoflavones genistein, daidzein, glycitin, puerarin, and biochanin A, and the flavonoids (±)-catechin, (-)-epicatechin, rutin, hesperidin, neohesperidin, quercitrin, and hesperetin in human urine. Urine samples were incubated with β-glucuroni...

A new furanoisoflavone, 2'-methoxy-4',5'-methylenedioxy-[2'',3'':7,8] furanoisoflavone, leptobotryanone (1), and a new natural O-prenylated isoflavone, 4'-γ,γ-dimethylallyloxy-5,7-dihydroxyisoflavone (2), were isolated from the twigs of Millettia leptobotrya, together with twelve known flavonoids, 4'-γ,γdimethylallyloxy-5-hydroxy-7-methoxyisoflavone (3), 2',6,7-trimethoxy-4',5'-methylenedioxyis...

The objective of the Vegetal Estrogens in Nutrition and the Skeleton (VENUS) project was to evaluate existing data on dietary exposure to compounds with oestrogenic and anti-oestrogenic effects present in plant foods as constituents or contaminants, and to identify and disseminate in vitro and in vivo methodologies to analyse the effects of such compounds on bone. To permit the assessment of ex...

A grazing experiment was carried out to study the concentration of phyto-oestrogens in herbage for cattle and in milk during two periods (May and June). Forty-eight Danish Holstein cows were divided into four groups with four treatment diets; white clover, red clover, lucerne and chicory-rich pastures. Each experimental period lasted 15 days. Herbage samples from the first day and individual mi...

Since the publication of the original paper, the authors have noticed an error in the primer sequences. The correct primer sequences are as follows. “Osterix (NM 001037632): forward: 5-cccgtctgactttgccccc-3, reverse: 5-ctgccccactgcctgcatat3 (203 bp); ALP (NM 013059): forward: 5-tggacggtgaacgggagaac-3, reverse: 5-cagagctggcccaggcaca-3 (238 bp); collagen type I (NM 053304):forward: 5-agcctggacttc...

Background: Biochanin A is an excellent dietary isoflavone that has the concomitant function of both medicine and foodstuff. The attenuation biochanin on blood-brain barrier (BBB) damage induced by cerebral ischemia-reperfusion remains unclear. Methods: C57BL/6 mice were subjected to 1 h middle artery occlusion (MCAO) followed 24 reperfusion. infarct volume brain was stained TTC, ...