In the present study, fresh waterfish Cirrhinus mrigala has been used as a model organism to understand mechanistic link between electroplating industrial effluent (EIE) and oxidative stress in aquatic ecosystems. To this end, fishes were exposed effluent, tank water for 10, 20 30 days under controlled laboratory conditions. Various hematological parameters (Total RBC WBC, Hemoglobin, MCV, MCH,...

This study aimed to diagnose Lumpy skin desease by isolation and identification of LSD from suspected naturally infected cattle its effect on immuno-biochemical parameters, inflammatory oxidative stress markers. The was conducted 25 cattle, 1-3 years age two cities in the Sharkia governorate. Ten clinically healthy represented control group fifteen diseased cases group. clinical signs showed an...

Abstract The color of flowers is one the main characteristics adopted for plants to attract pollinators ensure reproductive success plant, they are also important in their ornamental appeal Narcissus plant. In this study, we identified a NtMYB12 locus encoding an R2R3-MYB transcription factor. Comparative transcriptome analysis loss- and gain- tissue relative wild-type narcissus showed was main...

This work was done to evaluate the effect of ethanolic extract Palisota hirsuta on nephrotoxicity induced by carbontetrachloride (CCl4). Biochemical analyses were carried out determine levels creatinine, urea, total protein, chloride, carbonate using assay kits (products Randox Laboratories Crumlin and Teco Diagnostics), sodium, potassium (method Tietz,) malondialdelyde (MDA) level (thiobarbitu...

The Syrian hamster embryo (SHE) cell transformation assay is widely used to screen chemicals for carcinogenic potential. However, the biochemical mechanisms of transformation in SHE cells are incompletely understood relative to other rodent systems. Thus identification of proteins which change during transformation can provide clues to biochemical mechanisms. Previously, we published a map of S...

Background: Campylobacter infections may lead to serious conditions, including septicemia or other invasive forms of the disease, which require rapid and accurate laboratory diagnosis and subsequently appropriate antimicrobial therapy. The aim of this study was to compare the species distribution and antimicrobial susceptibility pattern of Campylobacter spp. strains isolated from patients and f...

Dadiah is a traditional dairy food from West Sumatra. made by fermenting fresh buffalo milk in banana leaves covered bamboo sticks at room temperature for two days. nutritious because it contains lactic acid bacteria (LAB) that have probiotic properties. This study aims to determine the characteristics and biochemical properties of LAB catalase assay fermentation type. We identified found dadia...

The reliability of an immunohistological method, applied to paraffin wax sections, was assessed for determination of oestrogen receptor content of biochemically oestrogen receptor negative breast carcinomata. Sixty consecutive tumours with oestrogen receptor concentrations of less than 10 fmol/mg cytosol protein, as estimated by dextran-coated charcoal biochemical assay, were examined. Paraffin...

In the course of a study of circadian rhythmicity in the number of cells in the S-phase of the cell cycle in mouse kidney, a comparison was made between 2 methods of estimation of DNA synthetic activity. One of the methods used was an autoradiographical analysis of the tritiated thymidine labelling index; the other was a biochemical assay of the DNA content of the tissues coupled with liquid sc...

background and objectives: numerous procedures in biology and medicine require the counting of cells. direct enumer- ation of colony forming units (cfus) is time-consuming and dreary accurate cell counting on plates with high numbers of cfus is error prone. in this study we report a new indirect cell counting method that was developed based on the use of redsafe fluorometric assay. the usefulne...