Abstract The present study was aimed to evaluate the moderating properties of lavender ( Lavandula officinalis ) essential oil (LEO) against immunotoxic effects organophosphate pesticide, malathion in rainbow trout, Oncorhynchus mykiss . For this purpose, fish were supplemented with LEO at dietary concentrations 0.5, 1, 2 and 4 ml/kg diet for 56 days. A non-LEO group also considered as control....

Introduction: L-ascorbic acid (vitamin C) is the most abundant intracellular antioxidant and an essential co-factor for the biosynthesis of many important biochemicals. Intracellular levels of ascorbic acid (AA) are remarkably high, especially in tissues such as brain and tumor cells, where concentrations may reach 10-30 mM. In this study, we show that native AA in solution is capable of produc...

Accelerator Mass Spectrometry is a mass spectrometric method of detecting long-lived radioisotopes without regard to their decay products or half-life. The technique is normally applied to geochronology, but is also available for bioanalytical tracing. AMS detects isotope concentrations to parts per quadrillion, quantifying labeled biochemicals to attomole levels in milligram-sized samples. Its...

inhibiting subtilisin BPN'. This paper describes purification and characterization of a subtilisin inhibitor (FMSI) from foxtail millet grain. Protein was measured by the method of Lowry et al.2) using bovine serum albumin as a standard. Protein concentrations of enzyme solutions were measured spectrophotometrically with extinction coefficients as described previously.3) The concentration of St...

Concerns about sustainability of fossil fuels and global warming, along with energy security, have provided a strong incentive in recent times towards research and development of carbon neutral alternatives including biofuels and biochemicals. This necessitates the development of efficient “biocatalysts” which can access wide range of products currently derived from petroleum. Current work deal...

Fourteen spot tests and color reactions were selected for their potential applicability to the problem of identifying urinary components separated by a high-resolution analytical system. These tests were screened with an inventory of 74 biochemicals of current interest. The results of this testing program are given and reveal the lack of specificity inherent in many tests which are sometimes as...

Biochemicals 5-hydroxytrptophan, 5-hydroxy indole acetic acid, ketanserin, bicuculline, baclofen, atropine, sodium octyl sulfonic acid, ethylene diamine tetra acetic acidEDTA, D-glucose, calcium chloride, bovine serum albumin fraction V, Superoxide dismutase from bovine erythrocytes were purchased from Sigma Chemical Co., St. Louis, USA. All other reagents were of analytical grade purchased loc...

General Experimental Procedures: Egg phosphatidylcholine (ePC) and routine chemicals were obtained from Sigma Chemical Co. SM-2 Bio-beads were purchased from Bio-Rad Laboratories. ULTROL grade Triton X-100 was from Roche Molecular Biochemicals. 1-acyl-2-C6-NBD-PC (NBD-PC) was from Avanti Polar Lipids. Salt-washed endoplasmic reticulum (SWER) was prepared from a rat liver homogenate as previousl...