Objectives- To isolate, culture-expand and differentiate mesenchymal stem cells from ovine bone marrow and determine their culture requirements for high expansion rate. Design- Experimental study. Animals- Five Shal sheep. Procedures- In this study, ovine marrow cells were plated and culture expanded through 3 successive subcultures. The resultant cells were then plated at differentiating condi...

background: during the resent years there has been interest in using bone marrow stem cells to treat liver cirrhosis. however, there is a potential concern for malignant transformation after stem cell therapy. the aim of this study was to evaluate the development of hepatocellular carcinoma (hcc) after autologous bone marrow stem cell transplantation for liver cirrhosis. methods: all the patien...

This investigation aimed to examine the protective function of vitamin E on rat bone marrow mesenchymal stem cells (MSCs) treated with cadmium chloride. Rat bone marrow MSCs were extracted using flashing-out and cultured in DMEM containing 10% FBS and 100 U/ml Pen/Strep. At the end of the third passage, cells were divided into 4 groups including control, cadmium chloride, cadmium chloride + vit...

Bone is maintained by coupled activities of bone-forming osteoblasts/osteocytes and bone-resorbing osteoclasts. Alterations in this relationship can lead to pathologic bone loss such as osteoporosis. It well known that osteogenic cells support osteoclastogenesis via production RANKL. Interestingly, our recently identified marrow mesenchymal cell population—marrow adipogenic lineage precursors (...

rabbits have the capacity to regenerate holes in their ears by forming a blastema, a tissue that is made up of a group of undifferentiated cells. the purpose of the present study was to isolate and characterize blastema progenitor cells and compare them with marrow mesenchymal stem cells (mscs). five new zealand white male rabbits were used in the present study. a 2-mm hole was created in the a...

introduction: as an inherited neurodegenerative disease, huntington's disease is accompanied with wide neuronal degeneration in neostriatum and neocortex. progress of the disease causes disabling clinical effects on movements, recognition and physiology of the body, and finally results in death. at this stage of knowledge we are, there is no effective therapeutic strategy for diminishing t...

human mesenchymal stem cells (hmscs), which also called mesenchymal stromal cells, are multipotent stem cell. human mscs typically are positive for the surface markers cd44, cd73, cd90, cd105, cd106, and also negative for hematopoietic markers cd34 and cd45.these cells can be isolated from postnatal bone marrow, adipose tissue, placenta, and scalp tissue, as well as from various fetal tissues. ...

Treatment with isolated allogeneic mesenchymal cells has the potential to enhance the therapeutic effects of conventional bone marrow transplantation in patients with genetic disorders affecting mesenchymal tissues, including bone, cartilage, and muscle. To demonstrate the feasibility of mesenchymal cell therapy and to gain insight into the transplant biology of these cells, we used gene-marked...