Melatonin is a unique multifunctional molecule that mediates reproductive functions in animals. In this study, we investigated the effects of melatonin on bovine parthenogenetic and androgenetic embryonic development, oocyte maturation, the reactive oxygen species (ROS) levels in parthenogenetic and androgenetic embryos and cumulus-oocyte complexes (COCs) hormone secretion with melatonin supple...

The present experiment was designed to identify possible effects of sera and steroid hormones added to a co-culture with bovine oviduct epithelial cells on embryonic development in vitro. Bovine oocytes were matured in vitro for 24 h and then fertilized in vitro using swim-up and heparin-treated, frozen-thawed spermatozoa. At 18 and 20 h after insemination, oocytes were cultured for 3 or 7 d in...

Abstract In bovine females, the production of embryos derived from oocytes obtained by ovum pick-up (OPU) is becoming a frequent procedure. Collection pre-pubertal animals enables shortening genetic distance, especially in high-value animals. Nevertheless, oocyte and later embryo developmental potential differ between prepubertal adult heifers. The aim this study was to determine possible under...

The early phase of embryonic development involves a lot signaling and growth factor proteins that will influence the interactions among cells. Administration BSA (Bovine Serum Albumin) at beginning embryo can interferes organogenesis causing abnormalities in development. Administrations were expected to induce responses immune system, which be hoped mimicking stress on system caused by infectio...

Real-time reverse transcription PCR has greatly improved the ease and sensitivity of quantitative gene expression studies. However, measurement of gene expression generally requires selection of a valid reference (housekeeping gene) for data normalization to compensate for inherent variations. Given the dynamic nature of early embryonic development, application of this technology to studies of ...

Oocyte maturation is known to affect the chances for successful fertilization, embryonic development, establishment of pregnancy and delivery of a live, healthy, and viable offspring. Two-photon laser scanning microscopy (TPLSM) has previously been used to evaluate early embryonic development without a detectable impairment of subsequent development, but has never been applied to assess mammali...