INTRODUCTION Methicillin-Resistant Staphylococcus aureus (MRSA) presenting reduced susceptibility to vancomycin has been associated to therapeutic failure. Some methods used by clinical laboratories may not be sufficiently accurate to detect this phenotype, compromising results and the outcome of the patient. OBJECTIVES To evaluate the performance of methods in the detection of vancomycin MIC...

Introduction:Serratia marcescens is a gram-negative pathogen of many species. Its pathogenicity and survival are linked to its capacity build biofilms as well strong inherent resistance antimicrobials cleaning agents. Objectives: To analyse the impact glyceryl trinitrate (GTN) on gene expression QS-related genes (rssB, rsmA,and pigP) S. marcescens. Methodology: The broth microdilution technique...

Twelve strains of Haemophilus influenzae were tested for susceptibility to gentamicin, ampicillin, chloramphenicol and cephalothin using two methods, agar dilution and microdilution (broth). Although inocula and incubation conditions were standardized, significant differences in the minimal inhibitory concentration (MIC) were seen as a result of growth media. Method dependent differences were a...

objective(s) rapidly growing mycobacteria (rgm) are capable of producing diseases in humans. since mycobacteria vary in their susceptibility, precise identification is critical for adoption of correct drug therapy. the main aim of this study was molecular identification and evaluation of antimicrobial susceptibility pattern of iranian clinically isolated myocbacterium fortuitum. materials and m...

The in vitro activities of 39 antimicrobial agents were assessed versus 74 clinical isolates of Branhamella catarrhalis. Resistance was observed only with penicillin and ampicillin and then only with beta-lactamase-producing strains. The results of in vitro susceptibility tests with agar dilution and broth microdilution procedures were found to be comparable. The results of broth tube macrodilu...

Several Flavobacterium species, comprising a heterogeneous group of gram-negative bacilli that are capable of causing opportunistic infections in humans, have recently been reclassified as Chryseobacterium or Myroides species. Intrinsically resistant to a number of antibiotics, these organisms have been reported to be susceptible to vancomycin and certain other drugs that are normally active ag...

Indirect drug susceptibility tests of Mycobacterium tuberculosis was done to investigate the accuracy and feasibility of a broth microdilution method (BMM) for determining minimal inhibitory concentrations of conventional drugs against M. tuberculosis. Test drugs included isoniazid (H), rifampicin (R), ethambutol (E), streptomycin (S) and pyrazinamide (Z). Fifty isolates of M. tuberculosis from...

A multicenter study was conducted to assess the inter- and intralaboratory reproducibility of the Etest for susceptibility testing of the rapidly growing mycobacteria. The accuracy also was evaluated by comparing Etest results to those obtained by broth microdilution. Ten isolates (four of the Mycobacterium fortuitum group, three of Mycobacterium abscessus, and three of Mycobacterium chelonae) ...

A multicenter study was conducted to expand the generation and analysis of data that supports the proposal of a reference method for the antifungal susceptibility testing of filamentous fungi. Broth microdilution MICs of amphotericin B and itraconazole were determined in 11 centers against 30 coded duplicate pairs of Aspergillus spp., Fusarium spp., Pseudallescheria boydii, and Rhizopus arrhizu...