Brucellosis is a worldwide zoonosis causing reproductive failures in livestock. It is shown that although the vaccine can prevent abortion, it does not provide complete protection against infection. So that vaccination of ewes with Rev.1 biotype can be a source of cattle and even human Brucellosis. The aim of this study was to evaluate possibility of Brucella cross-infection in cattle from Bruc...

Brucella species are important human and animal pathogens. Though, only little is known about mobile genetic elements of these highly pathogenic bacteria. To date, neither plasmids nor temperate phages have been described in brucellae. We analyzed genomic sequences of various reference and type strains and identified a number of putative prophages residing within the Brucella chromosomes. By in...

Assessing Brucella ceti Infections in Oregon and Washington Dolphins that Stranded with Histopathological Lesions Resembling Neurobrucellosis, 2006-2014 Tabitha George This thesis documents the presence of Brucella ceti in the Pacific white-sided dolphin (Lagenorhynchus obliquidens), striped dolphins (Stenella coeruleoalba), and shortbeaked common dolphins (Delphinus delphis) that stranded with...

Previous findings indicate that Brucella antigens and those from nonpathogenic alphaproteobacteria (NPAP) are cross-recognized by the immune system. We hypothesized that immunization with NPAP would protect mice from Brucella infection. Mice were immunized subcutaneously with heat-killed Ochrobactrum anthropi, Sinorhizobium meliloti, Mesorhizobium loti, Agrobacterium tumefaciens, or Brucella me...

In this study, a localized surface plasmon resonance (LSPR) nanobiosensor was designed to quantify anti-Brucella antibodies in the human sera. Smooth Lipopolysaccharide (LPS) extracted from Brucella melitensis via modified hot phenol water method and fixed on of gold nanoparticles by covalent interactions with functionalized nanoparticles. To obtain best performance probe, ratio LPS nanoparticl...

Brucella spp. are facultative intracellular parasitic pathogens that can survive and multiply in professional and nonprofessional phagocytes. These pathogens are responsible for brucellosis, which can cause abortion in domestic animals and undulant fever in humans. Brucella spp. can survive in a variety of cells and their virulence and chronic infections are thought to be due to their ability t...

The letter of Yang et al. (1) is an interesting account of the continuing issues associated with the misidentification of Brucella species by the current commercial identification systems. As mentioned in the letter, we experienced similar issues with the commercial bacterial identification assays that rely on phenotypic laboratory tests (2). These systems remain unreliable for identification o...

Monoclonal antibodies which bind Brucella A lipopolysaccharide (LPS)-specific, M LPS-specific, or cross-reactive epitopes were used as reagents in quantitative dot blot, Western blot (immunoblot), and immunoprecipitation analysis of Brucella whole cells, whole-cell extracts, and purified LPS preparations. This set of monoclonal antibodies detected four unique epitopes on Brucella LPS. The speci...

Brucella abortus is generally known to cause brucellosis in cattle and buffalo. Here, we report the draft genome sequence of Brucella abortus SKN 13, isolated from aborted cattle placenta in the area of Gujarat, India, providing precious resources for comparative genomic analyses of Brucella field strains.

Brucella melitensis is a facultative intracellular bacterium that causes brucellosis, the most prevalent zoonosis worldwide. The Brucella intracellular replicative niche in macrophages and dendritic cells thwarts immune surveillance and complicates both therapy and vaccine development. Currently, host-pathogen interactions supporting Brucella replication are poorly understood. Brucella fuses wi...