A patient relapsing with blastic lymphoid transformation of chronic myeloid leukemia after bone marrow transplantation received donor buffy-coat infusion. Low-dose chemotherapy was added because of a rapid WBC increase. Complete hematologic and cytogenetic remission was obtained. The patient remained in complete hematologic and cytogenetic remission for four months until he died in an accident....

Thirty acquired immunodeficiency syndrome patients with mycobacterial bacteremia documented by Du Pont Isolator (Du Pont Co., Wilmington, Del.) blood cultures underwent microscopic examination of buffy coat blood smears. Of 30 patients, 14 were culture positive for Mycobacterium avium-Mycobacterium intracellulare complex and 1 was positive for M. tuberculosis. Of 15 culture-positive patients, 1...

Three patients with hematologic relapse after bone marrow transplantation for chronic myelogenous leukemia were treated with interferon alpha and transfusion of viable donor buffy coat. All had complete hematologic and cytogenetic remission, which persisted 32 to 91 weeks after treatment. In two patients graft-versus-host disease developed and was treated by immunosuppression. These results are...

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Transfusi darah saat ini memegang peran medis yang penting, baik dalam terapi kegawat daruratan (life savings) maupun pada penyakit khusus memerlukan transfuse secara terus menerus. Komponen paling tinggi diminta unit pelayanan adalah Packed Red Cell (PRC), namun tidak sedikit dapat mengelami reaksi transfusi salah satunya febrile non haemolytic transfusion reaction (FNHTR). Penyebab utama dari...

the patient had visceral leishmaniasis (kala-azar), successfully treated with 30 intramuscular injections of sodium stibogluconate (SSG). Polymerase chain reaction showed Leishmania donovani DNA in a buffy coat specimen. Post-kala-azar dermal leishmaniasis (PKDL) is a chronic skin rash usually seen in apparently cured kala-azar patients in East Africa and South Asia. In the Indian subcontinent,...

We studied the sensitivity and specificity of PCR to detect T. gondii DNA by aliquoting various concentration of tachyzoites into laboratory specimens from 60 positive and 10 negative buffy-coat samples. We were able to detect the specific gene from purified DNA samples containing as few as 0.25 parasites per 100,000 human leukocytes. These results had an impressive initial 100% specificity but...