While the progress made in vitro culture of Chinese fir has produced satisfactory results, further improvements are warranted. To understand mechanism somatic embryogenesis (SE) fir, we conducted phenotypic observations, physiological and biochemical measurements, transcriptome analysis embryonic (EC) non-embryogenic callus (NEC) to provide a scientific basis for SE this species. We found that ...

AIMS Excessive alcohol consumption is associated with fracture non-union. Canonical Wnt pathway signaling activity regulates normal fracture healing. We previously demonstrated that binge alcohol exposure modulates β-catenin levels in the fracture callus of mice. Here, we sought to determine whether exogenous enhancement β-catenin signaling activity could restore normal fracture healing to bing...

The cactus Mammillaria gracillis Pfeiff. was propagated in vitro on Murashige and Skoog (MS) medium without any growth regulators. At the bases of some plants abundant callus masses developed. Lower agar and higher MS salt concentrations stimulated callus pro­ duction. In the culture, morphologically normal and hyperhydric shoots were regenerated. The growth of crown-gall tumour was induced on ...

background & aim: investigation of suspension cell culture and callus production in catharanthus roseus. catharanthus roseus is an herbaceous perennial plant belongs to the family apocynaceae. c. roseus is important as commercial medicinal plant because of its alkaloidal content. whole plant contains about 130 alkaloids those are classified as vinca alkaloids. vincristine and vinblastine; t...

White clover (Trifolium repens L.) callus tissue cultures accumulated the phytoalexin medicarpin after treatment with sulfhydryl reagents. After 24-hour exposures to sulfhydryl reagents, maximum obtainable levels of medicarpin, determined by high performance liquid chromatography analysis, were found with 50 millimolar N-ethyl maleimide, 25 millimolar HgCl(2), 2 millimolar p-chloromercuribenzoi...

Soybean Glycine max L. Merrill var. Amsoy 71 root callus tissue labeled with [1-(14)C]2,4-dichlorophenoxyacetic acid (2,4-D) which was subsequently incubated for 24 hours in the absence of 2,4-D, released considerable amounts of label into the media. These results led to an examination of the efflux of 2,4-D and 2,4-D metabolites during a 6-hour time period. Fifty% of the free 2,4-D was lost in...

Extracts from raw materials from different plant parts, tissue-cultured plants, and callus cultures of Oroxylum indicum were analyzed for in vitro antioxidant activities determined by DPPH radical scavenging assay and evaluated for phytochemical profiles by TLC and LC-MS methods. The results were analyzed by principal component analysis (PCA) to evaluate the similarity. Stalk, pedicel, flower, ...