نتایج جستجو برای: calyculin a
تعداد نتایج: 13431803 فیلتر نتایج به سال:
Although proteins phosphorylated on tyrosine residues can be enriched by immunoprecipitation with anti-phosphotyrosine antibodies, it has been difficult to identify proteins that are phosphorylated on serine/threonine residues because of lack of immunoprecipitating antibodies. In this report, we describe several antibodies that recognize phosphoserine/phosphothreonine-containing proteins by Wes...
We studied cellular phosphatase inhibitors, okadaic acid (OKA), calyculin A, and microcystin, on ENaC in A6 renal cells. OKA increased amiloride-sensitive current after about 30 minutes with maximal stimulation at 1-2 hours. Fluctuation analysis of cell-attached patches containing a large number of ENaC yielded power spectra with corner frequencies in untreated cells almost twice as large as in...
To identify protein kinases (PK) and phosphatases (PP) involved in regulation of the Na+-K+-2Cl-cotransporter in Ehrlich cells, the effect of various PK and PP inhibitors was examined. The PP-1, PP-2A, and PP-3 inhibitor calyculin A (Cal-A) was a potent activator of Na+-K+-2Cl-cotransport (EC50 = 35 nM). Activation by Cal-A was rapid (<1 min) but transient. Inactivation is probably due to a 10%...
BACKGROUND In migrating cells, the retrograde flow of filamentous actin (f-actin) from the leading edge toward the cell body is accompanied by the synchronous motion of microtubules (MTs, ), whose plus ends undergo net growth. Thus, MTs must depolymerize elsewhere in the cell to maintain polymer mass over time. The source and location of depolymerized MTs is unknown. Here, we test the hypothesi...
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