Liquid sheared amyloid-β (Aβ) initiates amyloid cascade reactions, producing unstable, potentially toxic oligomers. There is a need for new analytical tools with which to study these oligomers. A very small bore capillary flow system is proposed as a tool for studying the effects of liquid shear in amyloid research. This simple system consists of injecting a short cylindrical liquid sample plug...

Replacing pressure-driven flow with electroosmotic flow in chromatography with packed capillary columns results in a new and powerful analytical technique, combining the advantages of high-performance liquid chromatography (HPLC) and capillary electrophoresis (CE): capillary electrochromatography (CEC). In essence, it involves packing CE capillaries with HPLC stationary phases and applying a vo...

We study the impact of inlet channel geometry on microfluidic drop formation. We show that drop makers with T-junction style inlets form monodisperse emulsions at low and moderate capillary numbers and those with Flow-Focus style inlets do so at moderate and high capillary numbers. At low and moderate capillary number, drop formation is dominated by interfacial forces and mediated by the confin...

Inherent in the inflammatory response to sepsis is abnormal microvascular perfusion. Maldistribution of capillary red blood cell (RBC) flow in rat skeletal muscle has been characterized by increased 1) stopped-flow capillaries, 2) capillary oxygen extraction, and 3) ratio of fast-flow to normal-flow capillaries. On the basis of experimental data for functional capillary density (FCD), RBC veloc...

This work introduces capillary flow in paper microfluidics that features a flow rate Q that is constant in time, t, and independent of the viscosity of liquid sample, µ liquid : Q≠f(t, µ liquid). Compared to conventional paper microfluidics, we enclose the paper in solid walls and add a long and narrow air vent as outlet of the capillary pump, such that the flow rate is dominated by the downstr...

We present a new methodology using highly wetting porous titania films to develop microfluidic devices which can be employed for medical diagnostics. Capillary flows through thin porous media are attractive for small-scale liquid transport systems. Capillary flows show different characteristics depending on the medium, however, for most media it is challenging to realize arbitrary shapes and sp...

We investigate the rheological characteristics of human blood plasma in shear and elongational flows. While we can confirm a Newtonian behavior in shear flow within experimental resolution, we find a viscoelastic behavior of blood plasma in the pure extensional flow of a capillary breakup rheometer. The influence of the viscoelasticity of blood plasma on capillary blood flow is tested in a micr...

This study assesses the effect of the superoxide anion scavenger superoxide dismutase on myocardial capillary permeability-surface area (PS) products for small hydrophilic molecules after ischemia and reperfusion. Open-chest dogs underwent a 20-minute occlusion of the left anterior descending coronary artery followed by 1 hour of reperfusion. Myocardial plasma flow rate and capillary extraction...

This study assesses the effect of the superoxide anion scavenger superoxide dismutase on myocardial capillary permeability-surface area (PS) products for small hydrophilic molecules after ischemia and reperfusion. Open-chest dogs underwent a 20-minute occlusion of the left anterior descending coronary artery followed by 1 hour of reperfusion. Myocardial plasma flow rate and capillary extraction...

A streptavidin functionalized capillary immune microreactor was designed for highly efficient flow-through chemiluminescent (CL) immunoassay. The functionalized capillary could be used as both a support for highly efficient immobilization of antibody and a flow cell for flow-through immunoassay. The functionalized inner wall and the capture process were characterized using scanning electron mic...