abstract         background and objective: normal hemoglobin (hb) is formed of a heme group and a protein group known as globin. globin is made of four polypeptide chains and in hemoglobinopathies, the structure of one of these four polypeptide chain becomes abnormal. cellulose acetate method is a common way to differentiate haemoglobinopathies. inability to identify the components of hb low co...

The biopharmaceutical market is one of the fastest growing biotechnology markets. In order to ensure affordable and reliable therapeutics, process has be closely monitored. Capillary electrophoresis (CE) proven a valuable technique for analysis product concentration, critical quality attributes, process-related impurities, nutrients metabolites in cell culture medium. zone electrophoresis, capi...

To the Editor: We read with great interest the report “Interference of Radio-opaque Agents in Clinical Capillary Zone Electrophoresis” (1 ). A laboratory sent two similar patient samples for our evaluation. Both patients had received intravenous sodium meglumine ioxitalamate (Telebrix), a radioopaque contrast material that absorbs at 214 nm. The interfering substance was not visible on agarose ...

An improvement has been made to couple isoelectric focusing (IEF) sample injection and capillary zone electrophoresis in an untreated fused silica capillary. Electroosmotic flow is efficiently prevented by simply using a rubber block at the outlet end of the capillary during IEF sample injection. The experimental conditions that affect the concentration effect are discussed. A concentration enh...

• Capillary electrophoresis is well adapted for amino acids analysis. associated to fluorescence results in nanomolar concentration detections. conductimetry able be used most applications. mass spectrometry a very useful tool metabolomic Enantio-separations of are easily obtained using capillary electrophoresis.

Two steps of enzymatic oxidations from hypoxanthine to uric acid with xanthine oxidase (XOD) were kinetically analyzed by capillary electrophoresis/dynamic frontal analysis. When a substrate solution was introduced into separation buffer containing XOD, the reaction continuously proceeded during electrophoresis and product resolved zone. A plateau signal detected based on constant rate XOD. The...

The applicability of capillary electrophoresis for the analysis of four extensively used penicillin derivatives (benzylpenicillin, ampicillin, amoxicillin, oxacilllin) has been studied. Because of structural similarities, the electrophoretic behavior of these derivatives is very similar; consequently an efficient separation using the conventional capillary zone electrophoresis is hard to be ach...

The possibilities to use capillary zone electrophoresis (CZE) as a preparative tool have been studied, mostly from a theoretical point of view. The preparative performance of CZE is quantitated by the production rate, the amount of analyte that can be purified per unit of time. The production rate available with CZE is shown to depend in the first place on the chemical characteristics of the an...

Capillary zone electrophoresis was applied for the determination of amphetamine and related substances in seized drugs. A buffer made of 0.1 M phosphoric acid adjusted to pH 3.0 with triethanolamine was selected. With this background electrolyte, triethanolamine is adsorbed to the capillary wall and the electroosmotic flow is reversed. This gives rise to peaks with good symmetry, high efficienc...