In this work, a correlation study of monoclonal antibody-based enzyme-linked immunosorbent assays (ELISAs) and a liquid chromatography–electrospray mass spectrometric (HPLC/ESI/MS/MS) method for the determination of N-methylcarbamate insecticides carbofuran, carbaryl and methiocarb in fruit baby food is presented. The comparison of performance characteristics of the two methods was carried out ...

Fifteen pesticides were tested in laboratory bioassays on Galendromus occidentalis (Nesbitt), the principal phytoseiid mite predator in Washington apple orchards. We developed a rating system for pesticides using lethal and sublethal effects, and applied the rating system to our results. At the 1 dose, only spinetoram and lambda-cyhalothrin caused >75% acute mortality of females. Carbaryl, azin...

We previously found that carbamate pesticides induced significant apoptosis in human natural killer cells. To investigate whether carbamate pesticides also induce apoptosis in human T lymphocytes, in the present study Jurkat human T cells were treated in vitro with thiram, maneb, carbaryl or ziram. Apoptosis was determined by FITC-Annexin-V/PI staining. To explore the mechanism of apoptosis, in...

Mixtures of the pesticides carbaryl and thiabendazole were studied using solid phase spectrophosphorimetry at room temperature with Whatman No. 4 filter-paper as solid support and Pb(ii) as enhancer of the phosphorescence. The transmitted phosphorescence was used as an analytical signal and its measurement was performed by placing the paper containing the sample between two quartz plates. In th...

In spite of being widely used for in liquid biosensing applications, sensitivity improvement of conventional (5-20MHz) quartz crystal microbalance (QCM) sensors remains an unsolved challenging task. With the help of a new electronic characterization approach based on phase change measurements at a constant fixed frequency, a highly sensitive and versatile high fundamental frequency (HFF) QCM im...

Glutathione S-transferases (GSTs) play an important role in detoxification of xenobiotics in both prokaryotic and eukaryotic cells. In this study, four GSTs (LmGSTd1, LmGSTs5, LmGSTt1, and LmGSTu1) representing different classes were identified from the migratory locust, Locusta migratoria. These four proteins were heterologously expressed in Escherichia coli as soluble fusion proteins, purifie...

Amphibian populations are declining worldwide due to a number of stressors including pesticides and parasites. Conservation of these animals can be complicated because populations can differ dramatically in response to the same stressor. When consistently exposed to pesticides, some populations evolve tolerance through the process of natural selection acting across multiple generations. Alterna...

We investigated the regulation of the muscarinic cholinergic receptor (MChR) in brain from seven species of fish, two surrogates and five threatened or endangered species exposed to a series of chemicals as a measure of compensatory response among species. Fish were classified as either cold water (rainbow trout-surrogate, apache trout, lahanton trout) or warm water (fathead minnow-surrogate, r...

Pesticide application in horticultural crops has recently multiplied to increase crop yields and boost economic return. Consequently, the effects of pesticides on soil organisms plant symbionts is an evolving subject research. In this short-term study, we evaluated glyphosate (herbicide) carbaryl (insecticide) okra biomass AMF root colonization both shade house field settings. An additional tre...