We describe mesendoderm morphogenesis during gastrulation in the frog Xenopus laevis and investigate the mechanics of these movements with tissue explants. When a dorsal marginal zone explant is plated onto fibronectin, the mesendoderm moves away from the dorsal axial tissues as an intact sheet. Mesendodermal cells within these explants display monopolar protrusive activity and radially interca...

Breast cancer is the leading cause of death in women worldwide. The search for novel compounds with antitumor activity, with less adverse effects and higher efficacy, and the development of methods to evaluate their toxicity is an area of intense research. In this study we implemented the preparation and culture of breast tumor explants, which were obtained from precision-cut breast tumor slice...

Primary culture of adipose tissue has often been used to investigate pharmacological and nutritional regulation of adipocyte gene expression. Possible alteration of adipocyte gene expression by primary culture on its own has not been explored in detail. In order to address this issue, explants were prepared from human subcutaneous adipose tissue recovered from plastic surgery and maintained for...

23 24 Coral cell cultures made from reef-building scleractinian corals have the potential to aid in the pursuit 25 of understanding of the cnidarian-dinoflagellate symbiosis. Various methods have previously been 26 described for the production of cell cultures in vitro with a range of success and longevity. In this 27 study, viable tissue spheroids containing host tissue and symbionts (coral ex...

BACKGROUND Developing a simple and efficient method of obtaining primary cultured VSMCs is necessary for basic cardiovascular research. MATERIAL AND METHODS The procedure of our new method mainly includes 6 steps: isolation of the aortic artery, removal of the fat tissue around the artery, separation of the media, cutting the media into small tissue blocks, transferring the tissue blocks to cel...

Effects of increasing cefotaxime, carbenicillin and timentin concentrations on the in vitro morphogenesis of Eucalyptus grandis explants were evaluated. It was observed that carbenicillin and timentin increased the frequency of explant regenerating calluses and decreased necrosis up to 600 mg L-1, and that cefotaxime behaved similarly up to 300 mg L-1. At this level there was an increasing freq...

An accumulated turbidity and the presence of many bacilli in the plasma surrounding lepromatous tissue culture explants has been emphasized by Timofejewsky (1) and by Suwo and Kin (2) as indicating the multiplication of free bacilli in the plasma itself. Since this inference seems unwarranted by the observations made during the preceding studies with tissue cultures, it is desired to explain wh...

A method for tissue culture of micropropagation aquatic plant Staurogyne repens (Nees) Kuntze has been developed. Different phytohormones were investigated to optimize the growth explants. The shoot tip explants S. placed in MS nutrient medium including BAP (0.30-1.50 mg/l) + TDZ (0.10 NAA and Kn mg/l). Shoot regeneration frequencies changed as 77.77-100% 66.66-100% cultivation augmented with B...

cardiac magnetic resonance imaging (cmri) is a well-established noninvasive imaging modality in clinical cardiology. its ability to provide tissue characterization make it well suited for the study of patients with cardiac diseases. we describe a multi-modality imaging evaluation of a 45-year-old man who experienced a near drowning event during swimming. we underline the unique capability of ti...

AIMS Cultured cardiac explants produce a heterogeneous population of cells including a distinctive population of refractile cells described here as small round cardiac explant derived cells (EDCs). The aim of this study was to explore the source, morphology and cardiogenic potential of EDCs. METHODS Transgenic MLC2v-Cre/ZEG, and actin-eGFP mice were used for lineage-tracing of EDCs in vitro a...