The application of the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system of Streptococcus pyogenes (SpCas9) is currently revolutionizing genome engineering in plants. However, synthetic plant biology will require more complex manipulations of genomes and transcriptomes. The simultaneous addressing of different specific genomic sites with independent enzyme activities...

BACKGROUND AND PURPOSE Some proteins involved in self-repair after stroke in the adult brain are primarily expressed during embryonic development and strongly down-regulated during the early postnatal phase. Neuronal precursor cell-expressed, developmentally down-regulated gene (Nedd) 9 was recognized to be identical to Crk-associated substrate lymphocyte type (Cas-L), a docking protein that as...

Ovarian cancer is one of the most important causes of death from gynecological malignancies in Poland. Recent investigations took a note of possible relationship between tumor histological grading and immunoexpression of apoptosis and proliferation related proteins in serous ovarian cancers. The aim of the study was to assess the immunoexpression of CAS protein in serous ovarian tumors of diffe...

CRISPR-Cas (Clustered regularly interspaced short palindromic repeats-CRISPR-associated proteins) is a prokaryotic immune system that destroys foreign nucleic acids in a sequence-specific manner using Cas nucleases guided by short RNAs (crRNAs). Staphylococcus epidermidis harbours a Type III-A CRISPR-Cas system that encodes the Cas10-Csm interference complex and crRNAs that are subjected to mul...

Prokaryotes immunize themselves against transmissible genetic elements by the integration (acquisition) in clustered regularly interspaced short palindromic repeats (CRISPR) loci of spacers homologous to invader nucleic acids, defined as protospacers. Following acquisition, mono-spacer CRISPR RNAs (termed crRNAs) guide CRISPR-associated (Cas) proteins to degrade (interference) protospacers flan...

Sur 2601 admissions, chez 113 enfants at­teints de la maladie de Bouillaud, hospitalises en cinq ans dans deux services de pediatrie de Te­heran (C.H.U. de Pahlavi, C.H.U. de Logman­doleh Adham) 6 cas de manifestations cutanees  (trois cas de nodules, trois cas d'erytheme mar­ginatum) ont ete constates, trois d'entre eux souffrant d'une cardite severe et evolutive.  En Iran !'incidence des si...

C RISPR-Cas9 emerged as a powerful and universal technology for genome engineering with wide-ranging innovative implications across biology and medicine. The technology is based on RNAprogrammable molecular scissors to perform precise surgery on genes. Various versions of the system have been developed to broaden its range of applications to manipulate genes and their expression in a large vari...

At mitosis, focal adhesions disassemble and the signal transduction from focal adhesions is inactivated. We have found that components of focal adhesions including focal adhesion kinase (FAK), paxillin, and p130(CAS) (CAS) are serine/threonine phosphorylated during mitosis when all three proteins are tyrosine dephosphorylated. Mitosis-specific phosphorylation continues past cytokinesis and is r...

Src family kinases (SFKs) are modular signaling proteins possessing SH3, SH2, and tyrosine kinase domains. The SH3 and SH2 domains of SFKs have dual roles: they regulate the activity of the kinases, and they also target SFKs to their cellular substrates. We generated a series of novel SFKs by replacing the SH2 and SH3 domains of Hck with the syntrophin PDZ domain. In some constructs, the negati...

CRISPR (cluster of regularly interspaced palindromic repeats) is a prokaryotic adaptive defence system, providing immunity against mobile genetic elements such as viruses. Genomically encoded crRNA (CRISPR RNA) is used by Cas (CRISPR-associated) proteins to target and subsequently degrade nucleic acids of invading entities in a sequence-dependent manner. The process is known as 'interference'. ...