The resistance of native and trypsin-treated [14C] glucose-labeled cell walls to degradation by lysozyme and human lysosomal enzymes was confirmed. In contrast, chemically N-acetylated cell walls undergo significant degradation by these enzymes in the pH range of 4.5 to 5.5 without prior removal of the group-specific carbohydrate. N-acetylation after removal of the group A carbohydrate by forma...

Mature plant cell walls lose their ability to expand and become unresponsive to expansin. This phenomenon is believed to be due to cross-linking of hemicellulose, pectin, or phenolic groups in the wall. By screening various hydrolytic enzymes, we found that pretreatment of nongrowing, heat-inactivated, basal cucumber (Cucumis sativus) hypocotyls with pectin lyase (Pel1) from Aspergillus japonic...

responded to those of proteins deduced from the CR6RP1 and -2 cDNAs. Immunohistochemical staining revealed that the CRGRPs accumulEted specifically in the lignified walls of metaxylem vessels in the root, stein and leaf and in the lignified cell walls of periyascu]ar fibers in cucumber stems. ImmunostHining was also detected in the walls of metaxylem yessels and in the ceLl walls of adjacent sc...

Amino acid, carbohydrate, and lipid components of cell walls of Brucella abortus strain 19A (low virulence) and strain 2308 (high virulence) were compared by thin layer chromatography (TLC) and by use of an amino acid analyzer. A total of 15 amino acids were detected by both chromatographic methods. Each amino acid was present in greater amounts in strain 2308 than in strain 19A when equal amou...

In order to study the effects of structural variability, nanoindentation experiments were performed in Norway spruce cell walls with highly variable cellulose microfibril angle and lignin content. Contrary to hardness, which showed no statistically significant relationship with changing microfibril angle and lignin content, the elastic modulus of the secondary cell wall decreased significantly ...

Relatively large-scale production and purification of meningopneumonitis organisms was developed for chemical and immunological studies on cell walls of the infectious dense forms. By disruption of purified organisms with glass beads in a Mickle shaker, highly purified preparations of cell walls were obtained by sucrose density gradient centrifugation, enzyme digestion, and sodium dodecyl sulfa...

Throughout their life, plants typically remain in one location utilizing sunlight for the synthesis of carbohydrates, which serve as their sole source of energy as well as building blocks of a protective extracellular matrix, called the cell wall. During the course of evolution, plants have repeatedly adapted to their respective niche, which is reflected in the changes of their body plan and th...

cell walls prepured from its mycelia. RSC-a inhibited . more strong}y the re-extension of the hyphae, containing mainly mature cells, than RSC-c did. Upon incubation of the fungus vvith fluorescent chitinases, FITC}abeled RSC-a was found to be located in the hyphal tips, lateral walls, and septa, whi]e FITC-labeled RSC-c was only in the hyphal tip. RSC-a had a greater aMnity for the cell walls...

Foster, John W. (University of Georgia, Athens) and Edgar Ribi. Immunological role of Brucella abortus cell walls. J. Bacteriol. 84:258-268. 1962-Cell walls and protoplasm were prepared from organisms disrupted in a refrigerated pressure cell. Cell walls were purified by sedimentation in a linear glycerol gradient. Antigens capable of protecting mice against infection with Brucella abortus and ...

A new hydration cell has been constructed that allows wet biological samples, or samples of controlled moisture content, to be analysed in situ using infra-red microspectroscopy. The cell has been used to show that there are minor spectral changes associated with the hydration of pectin and tomato pericarp cell walls and slightly more significant changes in onion and carrot epidermal walls. The...