Correspondence to Takanobu Otomo: [email protected]; or Thomas Braulke: [email protected] T. Otomo’s present address is Department of Genetics, Osaka University Graduate School of Medicine, 5650871 Suita, Osaka, Japan. Abbreviations used in this paper: Fuc, -fucosidase; Man, -mannosidase; Gal, -galactosidase; Hex, -hexosaminidase; CFSE, carboxyfluorescein succinimidyl ester; Cts,...

BACKGROUND Peanut allergy is a life-threatening condition; there is currently no cure. While whole allergen extracts are used for specific immunotherapy for many allergies, they can cause severe reactions and even fatalities in peanut allergy. OBJECTIVE To identify short, HLA-degenerate CD4(+) T cell epitope-based peptides of the major peanut allergen Ara h 1 that target allergen-specific T c...

Muscle precursor cells may act not only as a means of inserting normal genes into diseased muscle fibres, in order to correct or alleviate a genetically inherited myopathy, but recent demonstrations have shown they may prove an invaluable tool for the expression of, and systemic dissemination of, non-muscle gene products. If muscle precursor cells are proved to act as such widespread vectors in...

Conventional assays for quantification of allo-reactive T-cell precursor frequencies (PF) are relatively insensitive. We present a robust assay for quantification of PF of T-cells with direct donor-specificity, and establish the kinetics of circulating donor-specific T cells after liver transplantation (LTx). B cells from donor splenocytes were differentiated into professional antigen-presentin...

Tanshinone IIA (TSA) is a widely used traditional Chinese medicine, which has been demonstrated to protect damaged liver cells and is currently administered in the treatment of liver fibrosis. Liver precursor cells, also termed oval cells, are key in the repair of liver tissues following injury. However, whether TSA improves the function of liver cells and protects the liver from injury by enha...

The murine local lymph node assay (LLNA) is a method for the prospective identification of chemical contact allergens. The current validated protocol assesses lymphocyte proliferation induced in the draining lymph node as a function of in situ incorporation of radiolabeled thymidine. We have explored the potential utility of an alternative nonradioisotopic marker of cell division, the cytoplasm...

Pharmacognosy Research,2021,13,3,165-172.DOI:10.5530/pres.13.3.9Published:June 2021Type:Original ArticleAuthors:Xingguo Quan, Ji-Young Lee, Jin Hee Park, Md. Masudul Haque, Yeon Kim, Ilhwan Anbok Il-Whan Choi, and SaeGwang Park Author(s) affiliations:Xingguo Quan1, Lee2, Park1, Haque1, Kim3, Kim4, Lee3, Choi1, Park1,* 1Department of Microbiology Immunology, College Medicine, Inje University, Bu...