OBJECTIVE Recently, we have shown that blocking of junctional adhesion molecule-1/A (JAM-1/A) inhibits basic fibroblast growth factor (bFGF)-induced angiogenesis. Because the process of endothelial cell proliferation is a key initial step of neovascularization, we studied the effect of functional knockdown of JAM-1 on human umbilical vein endothelial cell (HUVEC) adhesion and migration induced ...

Junctional adhesion molecule-A (JAM-A) is an adherens and tight junction protein expressed by endothelial and epithelial cells. JAM-A serves many roles and contributes to barrier function and cell migration and motility, and it also acts as a ligand for the leukocyte receptor LFA-1. JAM-A is reported to contain N-glycans, but the extent of this modification and its contribution to the protein's...

Atherosclerosis, caused in part by monocytes in plaques, continues to be a disease that afflicts the modern world. Whilst significant steps have been made in treating this chronic inflammatory disease, questions remain on how to prevent monocyte and macrophage accumulation in atherosclerotic plaques. Junctional Adhesion Molecule C (JAM-C) expressed by vascular endothelium directs monocyte trans...

Proinflammatory cytokines such as TNF-alpha and IFN-gamma induce cell adhesion molecules in endothelial cells and promote transmigration of leukocytes across endothelial cells. However, when those two were administered together, leukocyte transmigration paradoxically decreased. We cloned a human and bovine homologue of the junctional adhesion molecule (JAM), a novel molecule at the tight juncti...

Junctional adhesion molecule (JAM) is involved in tight junction (TJ) formation in epithelial cells. Three JAMs (A, B, and C) are expressed in rat hepatocytes, but only rat JAM-A is present in polarized WIF-B cells, a rat-human hepatic line. We used knockdown (KD) and overexpression in WIF-B cells to determine the role of JAM-A in the development of hepatic polarity. Expression of rat JAM-A sho...

Junctional Adhesion Molecule-1 May Have a Wider Role in Cardiovascular Disease To the Editor: Junctional adhesion molecule-1 (JAM-1; or F11 receptor) was first discovered as a surface protein on human platelets.1 It was later recognized as a receptor in the tight junction between cells involved in leukocyte migration in inflammation and platelet adhesion in thrombosis. The recent study by Waki ...

Neutrophil (PMN) transepithelial migration is dependent on the leukocyte beta(2) integrin CD11b/CD18, yet the identity of epithelial counterreceptors remain elusive. Recently, a JAM protein family member termed JAM-C was implicated in leukocyte adhesive interactions; however, its expression in epithelia and role in PMN-epithelial interactions are unknown. Here, we demonstrate that JAM-C is abun...

Department of Pharmacology, Lagos State University College of Medicine, Ikeja, Lagos, Nigeria. Correspondence: T. A. John, Department of Pharmacology, Lagos State University College of Medicine, PMB 21266, Ikeja, Lagos. Email: [email protected] Tel: 234 7028338910 Abbreviations: SMC, Smooth muscle cell; CHAH, Chronic high altitude hypoxia; alpha SMA, alpha Smooth muscle actin; eNOS, endo...

Junctional adhesion molecule, JAM, is involved in tight junction (TJ) formation in epithelial cells. Three JAMs (A, B and C) are expressed in rat hepatocytes, but only rat JAM-A is present in polarized WIF-B cells, a rat-human hepatic line. We used knock-down (KD) and over-expression in WIF-B cells to determine the role of JAM-A in the development of hepatic polarity. Expression of rat JAM-A sh...