نتایج جستجو برای: chain antibody

تعداد نتایج: 451649  

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2013
Mark Merchant Xiaolei Ma Henry R Maun Zhong Zheng Jing Peng Mally Romero Arthur Huang Nai-ying Yang Merry Nishimura Joan Greve Lydia Santell Yu-Wen Zhang Yanli Su Dafna W Kaufman Karen L Billeci Elaine Mai Barbara Moffat Amy Lim Eileen T Duenas Heidi S Phillips Hong Xiang Judy C Young George F Vande Woude Mark S Dennis Dorothea E Reilly Ralph H Schwall Melissa A Starovasnik Robert A Lazarus Daniel G Yansura

Binding of hepatocyte growth factor (HGF) to the receptor tyrosine kinase MET is implicated in the malignant process of multiple cancers, making disruption of this interaction a promising therapeutic strategy. However, targeting MET with bivalent antibodies can mimic HGF agonism via receptor dimerization. To address this limitation, we have developed onartuzumab, an Escherichia coli-derived, hu...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 1995
M Mack G Riethmüller P Kufer

Construction of a bispecific single-chain antibody derivative is described that consists of two different single-chain Fv fragments joined through a Gly-Ser linker. One specificity of the two Fv fragments is directed against the CD3 antigen of human T cells and the other is directed against the epithelial 17-1A antigen; the latter had been found in a clinical trial to be a suitable target for a...

Journal: :Infection and immunity 1998
Y Shibata K Kurihara H Takiguchi Y Abiko

Hemagglutinin is a major glycoprotein of Porphyromonas gingivalis vesicles and likely confers the ability to adsorb and penetrate into host tissue cells. To protect this bacterial invasion, murine monoclonal antibody (MAb) Pg-vc, which inhibited the hemagglutinating activity, was prepared by using P. gingivalis vesicles as an antigen. Western blot analysis revealed that when both MAb Pg-vc and ...

2009
Haggag Salah Zein Kazutaka Miyatake

A mouse monoclonal antibody (mAb) prepared by hybridoma technology could recognize the Cucumber mosaic virus (CMV) coat protein. The Fab fragments (antigen-binding site, one complete light chain, and part of one heavy chain) genes encoding the light chain and the Fd (a monovalent antigen-binding fragment consisting of a complete light chain paired with the variable region and the first constant...

Journal: :Anticancer research 2007
Yun Lin Xiaoping Wu Yi Shen Pengcheng Bu Dongling Yang Xiyun Yan

BACKGROUND An anti-CD146 monoclonal antibody, AA98, has been identified as an inhibitor of tumor angiogenesis. To overcome the inherent immunogenicity of murine antibody as well as to facilitate immunotoxin construction, a single chain AA98 V(H)/L with three-domain fragments was constructed and expressed in mammalian cells. MATERIALS AND METHODS The genes of the AA98 heavy chain variable regi...

Journal: :Cancer research 1982
V Raso J Ritz M Basala S F Schlossman

The toxic subunit of ricin has been conjugated by a disulfide bound to a monoclonal murine antibody (J-5) specific for the common acute lymphoblastic leukemia antigen (CALLA) expressed on human lymphoblastic cells. Both the monoclonal antibody and ricin A chain retained their original biological activity after conjugation. The ricin A chain portion of this conjugate effectively inhibited protei...

2013
Foroozan Mokhtarian Marianne Pino William Ofosu - Appiah David Grob

A study of cell surface phenotypes of PBL of myasthenia gravis (MG) patients showed that their T cells had a significantly higher percentage of 4B4+ T cells (the helper/inducer subset) than ageand sex-matched controls. The PBL of MG patients proliferated significantly higher than those of normal subjects (NS) in response to the purified a chain of the acetylcholine receptor (AChR). Anti-AChR an...

2015
Lotte B. Westerhof Ruud H. P. Wilbers Debbie R. van Raaij Christina Z. van Wijk Aska Goverse Jaap Bakker Arjen Schots

Secretory IgA (sIgA) is a crucial antibody in host defense at mucosal surfaces. It is a promising antibody isotype in a variety of therapeutic settings such as passive vaccination and treatment of inflammatory disorders. However, heterologous production of this heteromultimeric protein complex is still suboptimal. The challenge is the coordinate expression of the four required polypeptides; the...

2011
Maria Cristina Nishiwaki-Dantas Mariza Toledo de Abreu Cynthia Mendonça de Melo Ivana Lopes Romero Rubens Belfort Matos Neto Paulo Elias Correa Dantas

OBJECTIVES To identify Chlamydia trachomatis via polymerase chain reaction and a direct fluorescent antibody assay in patients with vernal keratoconjunctivitis while comparing the efficacies of both tests for detecting Chlamydia trachomatis in these conditions. METHODS Conjunctival scraping samples were obtained from 177 patients who were divided into two groups: a vernal keratoconjunctivitis...

Journal: :Journal of molecular biology 2008
Jeffrey E Lee Ana Kuehne Dafna M Abelson Marnie L Fusco Mary Kate Hart Erica Ollmann Saphire

13F6-1-2 is a murine monoclonal antibody that recognizes the heavily glycosylated mucin-like domain of the Ebola virus virion-attached glycoprotein (GP) and protects animals against lethal viral challenge. Here we present the crystal structure, at 2.0 A, of 13F6-1-2 in complex with its Ebola virus GP peptide epitope. The GP peptide binds in an extended conformation, anchored primarily by intera...

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