When we used plasma containing EDTA as an anticoagulant, the growth of Lacto bacillus casei was inhibited in the folate microbiological assay involving use of 300 p.L of assay mixture in a 96-well microtiter plate (1-3). We postulated that this inhibition was caused by EDTA chelation of manganese, iron, and magnesium ions, all of which are essential for growth of L. casei (4, 5). The singlestre...

The animal assay of potential new iron-chelating agents is at present dependent on cumbersome and imprecise iron balance studies in hypertransfused rodents. We report the development of a radioisotope assay in intact rats based on the transient labeling by ferritin 59Fe of the main source of chelatable iron within hepatocytes. The isotope was maximally available to chelators during the first 6 ...

This study measured excretion of 11 minerals induced by EDTA chelation therapy in 25 adults (aluminum, cadmium, calcium, chromium, copper, iron, lead, magnesium, manganese, mercury, zinc). We administered 20 EDTA infusions (3 g, with magnesium and ascorbate) 1 to 2 weeks apart and measured 24-hr urinary excretions of minerals before treatment and after treatments 1, 10, and 20. A modest supplem...

Background:Continuous chelation can be defined as the concept of using a single mix weak chelator with NaOCL throughout entire root canal preparation procedure without causing reduction in antimicrobial and proteolytic activity NaOCL. Etidronic acid, also known "1-Hydroxyethylidene-1, 1-Bisphosphonate" HEBP, or HEDP, is soft biocompatible utilized direct combination sodium hypochlorite to form ...