نتایج جستجو برای: chemiluminescence method

تعداد نتایج: 1634362  

Journal: :Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan 2010
Kana Yamaguchi Tsuyoshi Murai Hikaru Yabuuchi Shu-Ping Hui Takao Kurosawa

Monovalent bile acids, such as taurine- and glycine-conjugated bile acids, are excreted into bile by bile salt export pumps (BSEP, ABCB11). Human BSEP (hBSEP) is physiologically important because it was identified as the gene responsible for the genetic disease: progressive familial intrahepatic cholestasis type 2 (PFIC-2). The evaluation of the inhibitory effect of hBSEP transport activity pro...

Journal: :Japanese circulation journal 2001
J Midorikawa K Maehara H Yaoita T Watanabe H Ohtani S Ushiroda Y Maruyama

To investigate the time course of superoxide generation in ischemia-reperfusion in the in-vivo rat lung, the present study used an enhanced chemiluminescence method with 2-methyl-6-[p-methoxyphenyl]-3,7-dihydroimidazo[1, 2-alpha]pyrazin-3-one (MCLA) as a specific probe. The right pulmonary artery was occluded for 120 min, followed by 90-min reperfusion. Chemiluminescence induced by MCLA was con...

Journal: :Luminescence : the journal of biological and chemical luminescence 2006
Shi-Lv Chen Yu Liu Hui-Chun Zhao Lin-Pei Jin Zhong-Lun Zhang Yan-Zhen Zheng

A simple electrogenerated chemiluminescence (ECL) analysis method for the determination of norfloxacin (NFLX) is reported. It is based on ECL produced by Na(2)SO(3), which is sensitized by the Tb-NFLX complex. The relative ECL intensity of the Tb(3+)-NFLX-Na(2)SO(3) system is proportional to the amount of NFLX. The optimized experimental conditions were investigated. The linear range and detect...

Journal: :Plant Physiology 1978

Journal: :TrAC Trends in Analytical Chemistry 2009

Journal: :analytical and bioanalytical chemistry research 0
maryam koohsarian department of chemistry, golestan university, gorgan, ir iran ali mokhtari department of chemistry, golestan university, gorgan, ir iran

abstract a simple and sensitive chemiluminescence (cl) method was developed for direct quantification of oxymorphone, a µ opioid agonist that is approximately 10 times more potent than morphine. in this method potassium permanganate in polyphosphoric acid was used as cl reagent. using this method, oxymorphone can be determined over the concentration ranges 13.5-337.8 ng ml-1 and 0.34-6.76 µg ml...

Journal: :Analytical sciences : the international journal of the Japan Society for Analytical Chemistry 2006
Jun-Feng Li Li-Fei Bai Ying-Hua Wang Hong-Yan Wang

The solid-phase chemiluminescence analysis of gold on the surface of an anion-exchange resin was studied. A method for the fast determination of gold using flow injection was established. The anion-exchange resin was used as an adsorbent of gold ion in the form of AuCl4-. The cation-exchange resin was used for the on-line separation of cations in the matrix. To obtain the best results, the prec...

Journal: :Analytical and Bioanalytical Chemistry 2008
Dimitra N. Stratis-Cullum Guy D. Griffin Joel Mobley Tuan Vo-Dinh

This paper reports the first intensified biochip system for chemiluminescence detection and the feasibility of using this system for the analysis of biological warfare agents is demonstrated. An enzyme-linked immunosorbent assay targeting Bacillus globigii spores, a surrogate species for Bacillus anthracis, using a chemiluminescent alkaline phosphatase substrate is combined with a compact inten...

Journal: :Clinical chemistry 1999
F Fini G Gallinella S Girotti M Zerbini M Musiani

BACKGROUND Quantitative PCR of viral nucleic acids can be useful clinically in diagnosis, risk assessment, and monitoring of antiviral therapy. We wished to develop a chemiluminescence competitive PCR (cPCR) for parvovirus B19. METHODS Parvovirus DNA target sequences and competitor sequences were coamplified and directly labeled. Amplified products were then separately hybridized by specific ...

Journal: :Clinical chemistry 1991
T V Stabler A L Siegel

A chemiluminescence immunoassay (CLI) for the direct measurement of aldosterone in serum was developed with aminobutylethyl isoluminol (ABEI) as the label. In this competitive assay the samples are incubated with sample, antibody, aldosterone-carboxymethyl oxime-ABEI, and paramagnetic particles coated with second antibody. After magnetic separation and washing, the samples are incubated with 20...

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