Epitope tagging is a method of expressing proteins whereby an epitope for a specific monoclonal antibody is fused to a target protein using recombinant DNA techniques. The aim of this study was to sub-clone the peroxisomal protein (PEP) cDNA into a mammalian expression vector leading to the formation of a  chimeric PEP-cDNA containing the FLAG epitope. The FLAG-PEP recombinant cDNA was construc...

Alphaviruses, such as Semliki Forest virus, normally enter cells by penetration from acidic organelles of the endocytic pathway. The virions are internalised intact from the cell surface before undergoing acid-induced fusion in endosomes. To investigate the possibility that endocytosis might play a role in delivering virions to specific sites for replication, we compared SFV infection of baby h...

DNA lesions, cytotoxicity, and cellular uptake of CaCrO4 were com pared in Chinese hamster ovary, in mouse embryo fibroblast C3H10T'/2, and in human osteosarcoma cells. The concentration of CaCrO4 that reduced colony formation by 50% was 2or 3-fold less in human osteo sarcoma cells than in C3H 10T'/2 or Chinese hamster ovary cells. Alkaline elution studies showed that CaCrO4 induced DNA single ...

background: the critical role of interleukin-7 (il-7) in homeostatic proliferation and t cell survival has made it a promising cytokine for the treatment of various clinical conditions, especially those associated with lymphopenia. methods: in the present study we expressed recombinant human interleukin-7 (rhil-7) in chinese hamster ovary (cho)-k1 cells. cho-k1 cells were stably transfected wit...

Mismatch repair of palindromic loops in the presence or absence of single-base mismatches was investigated in wild-type and mismatch-binding defective mutant Chinese hamster ovary cells. Recombination intermediates with a maximum heteroduplex DNA (hDNA) region of 697 bp contained a centrally located, phenotypically silent 12-base palindromic loop mismatch, and/or five single-base mismatches. In...

Data are presented indicating that the adhesion of Chinese hamster ovary cells (CHO) to films of denatured type I collagen occurs by a fibronectin-dependent mechanism, whereas the adhesion of these cells to 3-dimensional gels of native type I collagen fibres may occur by either a rapid, fibronectin-dependent mechanism or by a slower, fibronectin-independent mechanism, whereas the adhesion of th...