gibberellic acid (ga3) is a group of plant hormones identified in various plants. the aim of this study was to determine the effects of ga3 on sperm parameters and in vitro fertilization (ivf). fifty six adult male rats were divided into seven groups as, control, treatment and sham. following 15, 30 and 45 days of ga3 and methanol alcohol (ma) administration, rats were euthanized and epididymis...

background: heat shock protein a2 (hspa2) is correlated with sperm maturity and function. therefore, dysfunctional expression of this gene results in abnormal spermatogenesis. on the other hand, dna damage in spermatozoa is considered to be an important cause of male infertility, and the presence of sperm with dna fragmentation and chromatin abnormalities in human ejaculates is well documented,...

Histone post-translational modification (PTM) antibodies are essential research reagents in chromatin biology. However, they suffer from variable properties and insufficient documentation of quality. Antibody manufacturers and vendors should provide detailed lot-specific documentation of quality, rendering further quality checks by end-customers unnecessary. A shift from polyclonal antibodies t...

Genome-wide identification of transcription factor (TF) binding sites is pivotal to our understanding of gene expression regulation. Although much progress has been made in the determination of potential binding regions of proteins by chromatin immunoprecipitation, this method has some inherent limitations regarding DNA enrichment efficiency and antibody necessity. Here, we report an alternativ...

Data obtained by the sperm chromatin structure assay (SCSA) on spermatozoa from nine bulls were correlated with fertility, measured by heterospermic performance (-0.94, P less than 0.01) and by alternate tests of sperm quality, including motility, acrosome integrity, Sephadex filtration and morphology of spermatozoa (all significant at P less than 0.05 to P less than 0.01). The SCSA uses flow c...

In this study calf thymus chromatin was fractionated into active (S1 and S2) and inactive (P 2) chromatin. Then the interaction of an anthracyc1ine antibiotic, adriamycin, with these fractions was investigated employing absorption and difference UV Nis spectroscopy and SDS and agarose gel electrophoreses. The results suggest that the binding of adriamycin to the S 1 fraction is slight but ...

Chromatin accessibility, or the physical access to chromatinized DNA, is a widely studied characteristic of eukaryotic genome. As active regulatory DNA elements are generally ‘accessible’, genome-wide profiling chromatin accessibility can be used identify candidate genomic regions in tissue cell type. Multiple biochemical methods have been developed profile both bulk and at single-cell level. D...

Centromere sequences are not conserved between species, and there is compelling evidence for epigenetic regulation of centromere identity, with location being dictated by the presence of chromatin containing the histone H3 variant CENP-A. Paradoxically, in most organisms CENP-A chromatin generally occurs on particular sequences. To investigate the contribution of primary DNA sequence to establi...

Differentiation is an epigenetic process which is installed by changes of transcriptional programs over successive cellular divisions. A number of studies have reported the effects of biochemical modifications of chromatin (DNA and chromatin proteins) on the regulation of transcription. Although, these studies are able to explain how transcription of a given gene is regulated (toward activation...

differentiation is an epigenetic process which is installed by changes of transcriptional programs over successive cellular divisions. a number of studies have reported the effects of biochemical modifications of chromatin (dna and chromatin proteins) on the regulation of transcription. although, these studies are able to explain how transcription of a given gene is regulated (toward activation...