نتایج جستجو برای: chromatographic method

تعداد نتایج: 1641355  

Journal: :The Analyst 2016
Eva Tyteca Jean-Luc Veuthey Gert Desmet Davy Guillarme Szabolcs Fekete

This review summarizes the use of computer assisted liquid chromatographic method development for the analytical characterization of protein biopharmaceuticals. Several modes of chromatography including reversed-phase liquid chromatography (RPLC), ion exchange chromatography (IEX), hydrophobic interaction chromatography (HIC) and some perspectives are discussed. For all these chromatographic mo...

2014
N. Rajan N. Rajana

Background: The objective of present investigation was to develop simple, sensitive, specific, precise and accurate reversed phase high performance liquid chromatographic (RP-HPLC) method and subsequent validation of the method for the determination of R-isomer in Pregabalin. Method: The chromatographic method was carried out using Chiral pak ZWIX (+) column (250mm x 4.0mm i.d., 3μm particle si...

Journal: :Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie 1981
W R Külpmann M Oellerich

A gas chromatographic procedure for the simultaneous determination of carbamazepine, phenobarbital and phenytoin using SP 2510 DA as stationary phase is presented. The antiepileptic drugs are determined simultaneously without derivatisation under isothermal conditions by a flame ionisation detector. The gas chromatographic procedure can be easily mechanized. The coefficient of variation for the...

Journal: :medical laboratory journal 0
fahime mohammad abadi department of clinical biochemistry arezoo mirfazeli department of neonatology hossein zaeri department of neonatology, mojgan nejabat department of medicinal chemistry mahsa taherizadeh department of clinical biochemistry mohammad ariaie department of research and technology

abstract        background and objective: measurement of amino acids is an important tool for metabolic studies and evaluation of patients’ clinical condition. the aim of this study was to analyze the plasma amino acids using reverse phase high performance liquid chromatography techniques (rp-hplc) with pre-column derivatization by o-phthalaldehyde (opa) in combination with 3- mercaptopropionic...

2014
Naoto Furusawa

This paper describes a reserved-phase high-performance liquid chromatographic (HPLC) method for detecting malachite green (MG) and leuco-malachite green (LMG) using an isocratic toxic organic solvent/reagent-free mobile phase. Chromatographic separations were performed an Inertsil WP300 C4 with 0.02 mol/L octane sulfonic acid–ethanol mobile phase and a photodiode-array detector. The total run t...

2009
S. N. MENON A. SINGH

A simple, rapid and precise reverse-phase high performance liquid chromatographic method has been developed for the quantitative determination of piperine from Ayurvedic Polyherbal formulations Arkavati, Krvyadras and Marichyadi taila. Chromatographic analysis was carried out on cosmosil C18 column (150mm x 4.6mm, 5μm particle) with a mobile phase of methanol: water in the volume ratio of 70:30...

2014
Z. S. Hamad B. M. Yahya

A rapid and sensitive high performance liquid chromatographic method has been developed for the determination of diclofenac sodium in rat plasma. The assay was performed after liquid-liquid extraction with 1M orthophosphoric acid and a mixture of hexane:isopropyl alcohol. Chromatographic separations were performed on C18 stationary phase with a mobile phase composed of acetonitrile: deionised w...

2009
Isabela da Costa César Gerson Antônio Pianetti

Youden’s test is a reliable method to evaluate the robustness of analytical methods, by means of an experiment design which involves seven analytical parameters combined in eight tests. In the present study, we assessed the robustness of a chromatographic method to quantify lumefantrine in raw material samples, using Youden’s test. Hence, it was possible to determine the effect of each analytic...

Journal: :Journal of chromatographic science 2014
Santaji Nalwade Vangala Ranga Reddy

A sensitive, stability-indicating reversed-phase high-performance liquid chromatographic method is developed and validated for the quantitative determination of retapamulin in topical dosage form. The chromatographic separation is achieved by using a C18 column (XTerra RP 18 250 × 4.6 mm, 5 µm) at 30°C. The mobile phase comprises a mixture of 0.05M potassium dihydrogen phosphate buffer (pH 6.1)...

Journal: :Journal of chromatography 1985
U A Shukla P L Stetson W D Ensminger

Verapamil is a newly marketed antiarrhythmic drug. It has a significant presystemic elimination when administered orally and, therefore, intravenous therapy is often recommended. Optimum therapeutic effect is obtained when verapamil plasma concentrations are between 100 and 400 ng/ml. Several chromatographic procedures for the determination of verapamil in biological fluids have been described ...

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