نتایج جستجو برای: chromosomal integration

تعداد نتایج: 268172  

2017
K. Leenhouts

An integration vector, pORI13, was developed to screen in Lactococcus lactis for expression signals induced by changes in the environment and to assay transcriptional activity of genes in single copy. The plasmid carries a promoterless Escherichia coli lacZ gene preceded by a start codon, a lactococcal ribosome binding site, and a multiple cloning site. Chromosomal Sau3AI fragments of L. lactis...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2005
Hezi Shaked Cathy Melamed-Bessudo Avraham A Levy

Gene targeting, which is homologous recombination-mediated integration of an extra-chromosomal DNA segment into a chromosomal target sequence, enables the precise disruption or replacement of any gene. Despite its value as a molecular genetic tool, gene targeting remains an inefficient technology in most species. We report that expression of the yeast RAD54 gene, a member of the SWI2/SNF2 chrom...

Journal: :Journal of biotechnology 2002
Ofra Bechor Dana R Smulski Tina K Van Dyk Robert A LaRossa Shimshon Belkin

A set of genetically engineered Escherichia coli strains was constructed, in which the promoter of the fabA gene is fused to Vibrio fischeri luxCDABE either in a multi-copy plasmid or as a single copy chromosomal integration. The fabA gene codes for beta-hydroxydecanoyl-ACP dehydrase, a key enzyme in the synthesis of unsaturated fatty acids, and is induced when fatty acid biosynthesis pathways ...

Journal: :Applied and environmental microbiology 1990
K J Leenhouts J Kok G Venema

Derivatives of plasmids pBR322, pUB110, pSC101, and pTB19, all containing an identical fragment of lactococcal chromosomal DNA, were integrated via a Campbell-like mechanism into the same chromosomal site of Lactococcus lactis MG1363, and the transformants were analyzed for the stability of the integrated plasmids. In all cases the erythromycin resistance gene of pE194 was used as a selectable ...

Journal: :Stem Cell Research 2021

Autism is a complex neuropsychiatric disorder defined by significant challenges in communication skills and social behavior as well repetitive conduct interests. Recent advances stem cell technologies allow vitro modeling of the underlying molecular disease mechanisms. Using integration-free episomal plasmids, we have generated novel iPS line (SDUKIi006-A) from patient diagnosed with atypical a...

Journal: :Cell 2002
Astrid R.W. Schröder Paul Shinn Huaming Chen Charles Berry Joseph R. Ecker Frederic Bushman

A defining feature of HIV replication is integration of the proviral cDNA into human DNA. The selection of chromosomal targets for integration is crucial for efficient viral replication, but the mechanism is poorly understood. Here we describe mapping of 524 sites of HIV cDNA integration on the human genome sequence. Genes were found to be strongly favored as integration acceptor sites. Global ...

Journal: :Applied and environmental microbiology 2013
Kia Vest Petersen Jan Martinussen Peter Ruhdal Jensen Christian Solem

We present a tool for repetitive, marker-free, site-specific integration in Lactococcus lactis, in which a nonreplicating plasmid vector (pKV6) carrying a phage attachment site (attP) can be integrated into a bacterial attachment site (attB). The novelty of the tool described here is the inclusion of a minimal bacterial attachment site (attB(min)), two mutated loxP sequences (lox66 and lox71) a...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 1979
M Breindl J Doehmer K Willecke J Dausman R Jaenisch

The chromosomal integration site of the structural gene of Moloney murine leukemia virus (M-MuLV) in the genome of BALB/Mo mice was mapped genetically. These mice transmit the exogenous M-MuLV as an endogenous virus at a single Mendelian locus. Two independent experimental approaches were used: (i) Non-virus-producing fibroblasts prepared from homozygous BALB/Mo embryos were fused to Chinese ha...

نمودار تعداد نتایج جستجو در هر سال

با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید