The study was conducted to compare differences in vitro maturation media and their effect on subsequent embryo production. procedures were approved by the agricultural research council animal ethics committee (Ref no: APAEC 2020/05) Tshwane university of technology AREC 2021/08/004). Follicular fluid containing a suspension oocytes retrieved from ovaries through aspiration slicing techniques. g...

Background: Parthenogenetic activation of mammalian oocytes using artificial stimuli is commonly used in various reproductive bio-techniques. Calcium ionophore is known to elevate intracellular calcium levels in the cytoplasm of oocytes through the influx of calcium from extracellular spaces. Ethanol promotes a single intracellular Ca2+ increase of greater and longer amplitude than the initial ...

The unequal division of the CD blastomere at second cleavage is critical in establishing the second embryonic axis in the leech Helobdella, as in other unequally cleaving spiralians. When CD divides, the larger D and smaller C blastomeres arise invariantly on the left and right sides of the embryo, respectively. Here we show that stereotyped cellular dynamics, including the formation of an inte...

OBJECTIVE This study aimed to correlate male age with embryo morphokinetic parameters on D3 considering the timing and the exact moment of embryo cleavage. METHODS Time-lapse imaging was used to produce an ideal cleavage curve for the embryos analyzed. The percentage of embryos under the curve was analyzed and correlated with male age. RESULTS 32.6% of the embryos from patients aged 28-33 y...

BACKGROUND The two main complications associated with the use of assisted reproduction techniques, ovarian hyperstimulation syndrome and multiple pregnancies, could be eliminated by milder ovarian stimulation protocols and the increased use of a single embryo transfer (SET) policy. A retrospective, cohort study was performed in private infertility centre to evaluate the embryological and clinic...

The effect of supplementation of follicular fluid (FF) at 5.0% concentration in in vitro maturation (IVM) media on in vitro production of buffalo embryos was studied. Oocytes were aspirated from slaughter house derived ovaries and graded. Cultutarable quality oocytes were randomly cultured in IVM media with FF (n = 334) and without FF (n = 228) and subjected to maturation. The maturation rate, ...

Mouse embryos are able to use exogenous thymidine during cleavage stages in vivo if the thymidine is injected directly into the oviduct. DNA synthesis during cleavage occurs asynchronously and the intensity of DNA synthesis in the blastomeres of the same embryo varies. At all stages, the label in some nuclei is distributed unevenly, suggesting that some chromosomes may be heterochromatinized ve...

introduction: to evaluate and compare efficiency of sil-select with percoll to recover sperm with normal chromatin in morphology and also studyeffect of these parameters on fertilization, embryo quality and cleavagescore. materials and methods: assessment of semen parameters, protamine deficiency (cma3) and excessive histone (aniline blue staining) were carried out before and after sperm proces...

The aim of this study was to examine the possible morphological and morphometric changes resulting from vitrification of embryos at the cleavage stage. In this study, 30 mice early-cleavage embryos at different stages of cleavage, resulting from in vitro fertilization (IVF) techniques, were examined before and after vitrification. Digital images were taken from embryos before and after vitrific...