Desferrioxamines E (1), D2 (2), X1 (3), and X2 (4), four macrocyclic N-hydroxy-N-succinyl diamine-based siderophores, were produced efficiently by heterologous expression of a fusion biosynthetic gene cluster. This expression system consisted of three genes (mbsA-C) from marine metagenomic DNA and one gene (dfoC(C)) from the terrestrial bacterium Erwinia amylovora. The first three genes are fun...

Diverse cellular processes are driven by motor proteins that are recruited to and generate force on lipid membranes. Surprisingly little is known about how membranes control the force from motors and how this may impact specific cellular functions. Here, we show that dynein motors physically cluster into microdomains on the membrane of a phagosome as it matures inside cells. Such geometrical re...

Fusion protein technology represents the strategy to achieve rapid, efficient, and cost-effective proteinexpression. Epsilon and Beta toxins are the most potent Clostridial toxins and cause disease in animals.This study describes in silico fusion of Clostridium perfringens types D and B epsilon and beta toxin genesthat was used for cloning in E.coli. The etx and cpb genes were...

The limitation of energy supply is a crucial problem in wireless multimedia sensor networks. In this article, we research and optimize the energy utilization during data collection by adopting mobile agent nodes. First, we demonstrate that the energy consumption of the whole network is affected by the data correlation coefficient for a multimedia sensor network with a random node deployment. Th...

Recent experiments on the interaction of intense, ultrafast pulses with large van der Waals bonded clusters have shown that these clusters can explode with sufficient kinetic energy to drive nuclear fusion. Irradiating deuterium clusters with a 35 fs laser pulse, it is found that the fusion neutron yield is strongly dependent on such factors as cluster size, laser focal geometry, and deuterium ...

This is our second participation in the TREC Legal Track. The TREC Legal Track 2011 featured only the Learning Task. We participated in Topics 401 and 403. We used Lemur 4.11 for Boolean retrieval and followed it with a clustering technique, where we chose members from each cluster (which we called seeds) for relevance judgement by the TA and assumed all other members of the cluster whose seeds...

detection of brain tissues using magnetic resonance imaging (mri) is an active and challenging research area in computational neuroscience. brain mri artifacts lead to an uncertainty in pixel values. therefore, brain mri segmentation is a complicated concern which is tackled by a novel data fusion approach. the proposed algorithm has two main steps. in the first step the brain mri is divided to...

We consider distributed detection in a clustered wireless sensor network (WSN) deployed randomly in a large field for the purpose of intrusion detection. The WSN is modeled by a homogeneous Poisson point process. The sensor nodes (SNs) compute local decisions about the intruder’s presence and send them to the cluster heads (CHs). A stochastic geometry framework is employed to derive the optimal...

Spectra of coincident charged particles from the reactions induced by a 52 MeV 7 Li beam incident on a beryllium target were measured. Strong contributions of the 7 Li quasi-free scattering off the α-cluster in 9 Be nucleus were observed. This observation supports the conclusions from the study of complete fusion of weakly bound light nuclei at low energies that the " fragility " of the nuclei ...

The glycoproteins (G proteins) of vesicular stomatitis virus (VSV) and related rhabdoviruses (e.g., rabies virus) mediate both cell attachment and membrane fusion. The reversibility of their fusogenic conformational transitions differentiates them from many other low-pH-induced viral fusion proteins. We report single-virion fusion experiments, using methods developed in previous publications to...