نتایج جستجو برای: cma3 assay

تعداد نتایج: 221893  

2018
Alexandr Sember Jörg Bohlen Vendula Šlechtová Marie Altmanová Šárka Pelikánová Petr Ráb

Polyploidization has played an important role in the evolution of vertebrates, particularly at the base of Teleostei-an enormously successful ray-finned fish group with additional genome doublings on lower taxonomic levels. The investigation of post-polyploid genome dynamics might provide important clues about the evolution and ecology of respective species and can help to decipher the role of ...

2017
Ana Claudia Swarça Ana Lucia Dias Alberto Sergio Fenocchio

The objective of this study was to cytogenetically analyze Phractocephalus hemioliopterus comparing the findings with other data to infer relationships among Pimelodidae species. The results revealed a diploid number of 2n = 56 and the karyotype composed of 16 metacentric, 20 submetacentric, 6 subtelocentric and 14 acrocentric chromosomes (FN = 98). The Ag-NORs, 18S rDNA and CMA3 signals were c...

2016
Mahsa Nazar Ali Reza Talebi Mohammad Hosseini Sharifabad Abolghasem Abbasi Arezoo Khoradmehr Amir Hossein Danafar

BACKGROUND The particles in the range of 1-100 nm are called nanoparticles. Gold nanoparticle is one of the most important metal nanoparticles with wide usage. OBJECTIVE This study investigated the effects of gold nanoparticles on sperm parameters and chromatin structure in mice. MATERIALS AND METHODS In this experimental study, 72 male bulb-c mice were divided into 9 groups including: 4 Sh...

2011
Priscilla Cardim Scacchetti José Carlos Pansonato-Alves Ricardo Utsunomia Claudio Oliveira Fausto Foresti

Conventional (Giemsa, C-Banding, Ag-NORs, CMA3) and molecular (5S rDNA, 18S rDNA, telomeric sequences) cytogenetic studies were carried out in specimens of ten distinct fish populations of the genus Gymnotus (Gymnotus sylvius Albert and Fernandes-Matioli, 1999, Gymnotus inaequilabiatus Valenciennes, 1839, Gymnotus pantherinus Steindachner, 1908, and G. cf. carapo Linnaeus, 1758) from different ...

2013
Esmat Mangoli Ali Reza Talebi Morteza Anvari Majid Pourentezari

BACKGROUND Diabetes mellitus (DM), primary or idiopathic is a chronic disorder of the carbohydrate, lipid and protein metabolism. DM may impact male reproductive function at several levels. It is shown that DM has detrimental effects on sperm parameters in human and experimental animals. OBJECTIVE The aim of this study was to observe the effects of diabetes on sperm parameters (viability, cou...

2016
Tatiane Ramos Sampaio Larissa Bettin Pires Natália Bortolazzi Venturelli Mariana Campaner Usso Renata da Rosa Ana Lúcia Dias

The family Curimatidae is a fish group usually considered chromosomally conserved in their diploid number. However, some studies show small changes in the karyotype microstructure, and the presence of B chromosomes, indicating a chromosomal diversification within the group, even if structural changes in the karyotypes are not visible. Few studies associate this trait with an evolutionary patter...

Journal: :Human Reproduction 2023

Abstract Study question Does supplementation of human sperm media with L-Proline improve function and chromatin quality during in Vitro Incubation? Summary answer The inclusion L-proline as an antioxidant improves integrity probably by modulating oxidative stress What is known already Infertility affects millions people reproductive age globally. gametes one the most critical aspects that could...

Journal: :iranian journal of microbiology 0
ehsan khalili department of clinical biochemistry, school of medicine, tehran university of medical sciences, tehran, iran. vahid hosseini department of clinical biochemistry, school of medicine, tehran university of medical sciences, tehran, iran. roya solhi department of clinical biochemistry, school of medicine, tehran university of medical sciences, tehran, iran. mahdi aminian department of clinical biochemistry, school of medicine, tehran university of medical sciences, tehran, iran and recombinant vaccine research center, tehran university of medical sciences, tehran, iran.

background and objectives: numerous procedures in biology and medicine require the counting of cells. direct enumer- ation of colony forming units (cfus) is time-consuming and dreary accurate cell counting on plates with high numbers of cfus is error prone. in this study we report a new indirect cell counting method that was developed based on the use of redsafe fluorometric assay. the usefulne...

ژورنال: Anatomical Sciences Journal 2006
Mardani, Mohammad, Nasr Esfahani, Mohammad Hosein, Razavi, Shahnaz, Shiri, Reza,

Sperm premature chromosomal condensaion (PCC) and failed oocyte activation are considered to result in failed fertilization post intracytoplasmic sperm injection (ICSI). Recent studies also suggested that sperm protamine deficiency may induce PCC. The ain of this study was to assess the effect of failed oocyte activation and protamine deficiency on failed ferilization post ICSI and to indicate ...

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