A themophilic cellulase-producing bacterium was isolated from a hot spring district and identified as Geobacillus sp. HTA426. The cellulase enzyme produced by the Geobacillus sp. HTA426 was purified through ammonium sulfate precipitation and ion exchange chromatography, with the recovery yield and fold purification of 10.14% and 5.12, respectively. The purified cellulase has a molecular weight ...

Bacteria are important sources of cellulases with various industrial and biotechnological applications. In view of this, a non-hemolytic bacterial strain, tolerant to various environmental pollutants (heavy metals and organic solvents), showing high cellulolytic index (7.89) was isolated from cattle shed soil and identified as Bacillus sp. SV1 (99.27% pairwise similarity with Bacillus korlensis...

In this study, forty-two fungi from soil were isolated and tested for their carboxymethyl cellulase (CMCase) and xylanase activities. From all isolates, the fungal isolate FR60, which was identified as Aspergillus aculeatus Iizuka, showed high activities in both CMCase and xylanase with 517 mU/mg protein and 550 mU/mg protein, respectively. The crude enzyme from A. aculeatus Iizuka FR60 could h...

Out of 12 fungal strains, two fungal strains, namely Aspergillus flavus AT-2 and Aspergillus niger AT-3, were found to produce maximum cellulase production. The CMCase activities of A. flavus AT-2 and A. niger AT-3 under state solid fermentation (SSF) conditions were of 42.69 and 40.08%, respectively, which was higher than those obtained under submerged fermentation (SmF) conditions. A fungal i...

Benzene is a highly toxic organic contaminant having carcinogenic potential and a serious ground water contaminant. Refinery industry is a major source of benzene contamination in the environment. The present paper focuses on biodegradation of benzene under anaerobic condition by using enriched microbial consortium. Enriched bacterial isolates, identified by 16S rRNA sequencing technique were t...

The objective of this research was to investigate xylanase production by filamentous fungi (Trichoderma viride) to determine the best cultivation conditions in the process, aiming toward optimization of enzyme production. The best temperature, as well as the best carbon source, for biomass production was determined through an automated turbidimetric method (Bioscreen-C). The enzyme activity of ...