Citation: Sabet Zangeneh H, Mohammaddust Chamanabad HR, Zand E, Asghari A, Alamisaeid K, Travlos IS and Alebrahim MT (2016) Study of Fitness Cost in Three Rigid Ryegrass Populations Susceptible and Resistant to Acetyl-CoA Carboxylase Inhibiting Herbicides. Front. Ecol. Evol. 4:142. doi: 10.3389/fevo.2016.00142 Study of Fitness Cost in Three Rigid Ryegrass Populations Susceptible and Resistant t...

The mechanisms whereby growth hormone and dexamethasone modulate the stimulation of fatty acid synthesis by insulin in adipose tissue from lactating and non-lactating sheep have been investigated. Maintenance of adipose tissue from wethers (castrated male sheep) in tissue culture for 24 or 48 h with insulin resulted in an increased proportion of acetyl-CoA carboxylase being present in the activ...

ATP:citrate lyase (ACL) catalyzes the conversion of citrate to acetyl-coenzyme A (CoA) and oxaloacetate and is a key enzyme for lipid accumulation in mammals and oleaginous yeasts and fungi. To investigate whether heterologous ACL genes can be targeted and imported into the plastids of plants, a gene encoding a fusion protein of the rat liver ACL with the transit peptide for the small subunit o...

1. The effect of preincubation of extracts of lactating rat mammary gland with ATP, Mg2+ and micromolar concentrations of Ca2+ on the activity of acetyl-CoA carboxylase was studied. 2. Both Mg2+ and Ca2+ activated the enzyme. Activation with Mg2+ (5 mM) was larger than that with Ca2+ (calculated free Ca2+ concentration = 20-50 microM), but the activity decreased after reaching a peak. The activ...

Fatty acid synthesis is traditionally viewed as being confined to the cytosolic cellular fraction, although a substantial body of data indicates that both microsomes and mitochondria are capable of initiating fatty acid synthesis and may contain acetyl-CoA carboxylase [acetyl-CoA:carbon-doxide ligase (ADP-forming), EC 6.4.1.2], fatty acid synthetase, and ATP-citrate lyase [ATP citrate (pro-3S)-...

Enzymically inactive acetyl-CoA carboxylase [acetyl-CoA:carbon-dioxide ligase (ADP-forming), EC 6.4.1.2] was found as a component of bovine milk-fat-globule membrane (MFGM). Acetyl-CoA carboxylase was present in MFGM at a higher concentration than in cytosolic or mitochondrial fractions of bovine mammary tissue, which makes it unlikely that its presence was due to simple contamination by these ...

medium containing [” Plphosphate, the distribution of 32P-labelled acetyl-CoA carboxylase in the fractions can be investigated and it has been found that a greater proportion of the phosphorylated enzyme is present in the polymeric form after exposure of the intact tissue to insulin. Present studies are concerned with investigating whether this polymeric active form is phosphorylated to a great...

1. A new rapid method for the purification of fat-cell acetyl-CoA carboxylase is described; the key step is sedimentation after specific polymerization by citrate. 2. Incubation of epididymal fat-pads or isolated fat-cells with insulin or adrenaline leads to a rapid increase or decrease respectively in the activity of acetyl-CoA carboxylase measured in fresh tissue extracts. The persistence of ...

Peroxisome proliferator-activated receptor alpha (PPAR alpha)-null mice were used to investigate the nature of the relationship between the normal circadian rhythm of hepatic PPAR alpha expression and the expression of the lipogenic and cholesterogenic sterol regulatory element-binding protein (SREBP)-regulated genes, acetyl-CoA carboxylase, fatty acid synthase (FAS), and 3-hydroxy-3-methylglut...