This publication describes the straightforward and redox-neutral synthesis of 10-bromophenylethynylcobalamin its facile conversion to 10-bromoaquacobalamin. In this approach, phenylethynyl ligand acts as convenient light-stable protecting group that is removed in quantitative yield under acidic conditions. The proteolytic cleavage at pH 2.0 was studied pseudo-first-order conditions 1.5 times sl...

Species of the marine mussel family Mytilidae have two types of mitochondrial DNA: one that is transmitted from the mother to both female and male offspring (the F type) and one that is transmitted from the father to sons only (the M type). By using pair matings that produce only female offspring or a mixture of female and male offspring and a pair of oligonucleotide primers that amplify part o...

Recombinant DNA techniques have been used to quantitate the amount of nucleotide sequence divergence in the mitochondrial DNA population of individual normal humans. Mitochondrial DNA was isolated from the peripheral blood lymphocytes of five normal humans and cloned in M13 mphl; 49 kilobases of nucleotide sequence information was obtained from 248 independently isolated clones from the five no...