econazole nitrate (en), a synthetic compound, is now in use as a routine antifungal drug. en was shown to have antitumor effect, the tumor cell killing mechanisms, however, remain unclear. in this research, the apoptosis-inducing effect of en on mcf-7 cells was investigated. the results showed that en inhibited the proliferation of mcf-7 cells in a time- and dose-dependent manner by mtt method ...

To compare the clonogenicity and distribution of CD34+ subsets in bone marrow (BM), granulocyte colony-stimulating factor (G-CSF) mobilized peripheral blood (PB) and cord blood (CB), we analyzed in vitro colony formation and CD34+ cells co-expressing differentiation molecules (CD38, HLA-DR), myeloid associated molecules (CD13, CD33), a T-cell associated molecule (CD3), and a B-cell associated m...

Lung colony-forming ability of clones isolated from a fibrosarcoma was examined by i.v. injection of the cells cultured in vitro. The clones were heterogeneous in their lung colony-forming ability, demonstrating variability of this malignant characteristic.

Methylthioadenosine phosphorylase (MTAP) is important for the salvage of adenine and methionine. Recently, we found frequent deletion of MTAP in T-cell acute lymphoblastic leukemia (T-ALL) patients both at diagnosis and at relapse (A. Batova et al., Blood, 88: 3083-3090, 1996). In addition, MTAP deficiency has been reported in other cancers. Thus, MTAP deficiency in cancer may offer opportuniti...

Granulocyte macrophage colony-forming cells (GM-CFC) have the potential to develop into either macrophages and/or neutrophils. With a highly enriched population of these cells we have found that although GM-CFC are equally responsive to macrophage colony stimulating factor (M-CSF) and stem cell factor (SCF) in terms of DNA synthesis, M-CSF stimulated the development of colonies containing macro...

The influence of in utero low-dose ionizing radiation exposure on murine hemopoietic embryogenesis was investigated. In vitro assays such as micro plasma-clot cultures and doublelayer soft agar cultures served as sensitive biodosimeters to determine erythropoietic and granulopoietic injuries. Day-10-5, HA/ICR, pregnant mice were irradiated with 0, 50, 100, 150,200, or 300 rads, and day-14-5 fet...

conclusions caffeic acid treatment reduced bacterial growth and resulted in a decreased number of colony formation. the simultaneous effect of caffeic acid and laser at three time courses showed a synergic effect in reducing colony formation compared to the control and caffeic acid, and laser alone. objectives the aim of this study was to investigate the effect of caffeic acid and low-power las...