Colorimetry, 2 edition (4) contains all the definitions of colorimetric terms, discusses CIE colorimetric practice and provides many useful tables to perform colorimetric calculations. CIE Technical Committee TC 1-48 deals at the present moment with updating this publication. The update will not change anything basic in CIE colorimetry. It will mainly get the recommendations in line with the re...

(1) Background: d-alanine-d-alanine ligase (DdlA), an effective target for drug development to combat against Mycobacterium tuberculosis (Mtb), which threatens human health globally, supplies a substrate of d-alanyl-d-alanine for peptidoglycan crosslinking by catalyzing the dimerization of two d-alanines. To obtain a better understanding of DdlA profiles and develop a colorimetric assay for hig...

Although of the many colorimetric microestimations of sugar the Folin and Wu method (1920) has remained in most widespread use in clinical laboratory practice, the still increasing number of modifications continue to draw attention to several inherent weaknesses of the method which have not yet been eliminated, one of which is the instability of the colour solution. To avoid these drawbacks a m...

SINcE the publication of von Fellenberg's method [1923] for the determination of small quantities of iodine, a very large number of new methods and modifications have been described, some of which claim a surprising degree of accuracy. The multiplicity of methods is sufficient to show that none of them is really satisfactory. The von Fellenberg method includes two methods for the final determin...

A label-free aptamer-based assay for the highly sensitive and specific detection of Ochratoxin A (OTA) was developed using a cationic polymer and gold nanoparticles (AuNPs). The OTA aptamer was used as a recognition element for the colorimetric detection of OTA based on the aggregation of AuNPs by the cationic polymer. By spectroscopic quantitative analysis, the colorimetric assay could detect ...

We developed a method for the selective determination of 4-aminobenzoic acid by use of 4-aminobenzoate hydroxylase (EC 1.14.13.27) to convert 4-aminobenzoic acid to 4-hydroxyaniline. The subsequent conversion of 4-hydroxyaniline to indophenol dye was quantified colorimetrically. The method is reproducible, and the assay response is linear up to 40 mumol/L. Selectivity exceeding that of the curr...

A Bayesian method of generating color correction matrices for digital image sensors is presented. This method was developed for sensors with poor colorimetric quality, and uses strong prior assumptions about object surface reflectance functions to improve color correction accuracy. These assumptions are expressed through linear model constraints on surface reflectance coupled with a Normal dist...

An immunochemical method for quantitative analysis of the tartrate-resistant acid phosphatase (EC 3.1.3.2), band 5, is presented. This method involves precipitation of the enzyme from the serum by the antibody specific to band 5 and by sheep anti-rabbit immunoglobulin, followed by analysis of the enzyme activity in the precipitate. The precipitation procedure eliminates the interferences of the...

In this nonenzymatic colorimetric method, dichlorobenzoquinone chloroimine is used as a color reagent to measure uric acid in urine. This method is less subject to the interferences that make enzymatic methods unreliable and is inexpensive, simple, and fast.