Methanol has long being used as a substrate analogue to probe access pathways and investigate water delivery at the oxygen-evolving complex (OEC) of photosystem-II. In this contribution we study the interaction of methanol with the OEC by assembling available spectroscopic data into a quantum mechanical treatment that takes into account the local channel architecture of the active site. The eff...

We report the case of a patient referred to our prenatal diagnostic unit due to the suspicion of spina bifida at 24 weeks gestation. Morphological assessment revealed rachischisis associated with Chiari type II, single kidney and anorectal malformation. The nonvisualization of the anal sphincter complex (ASC) on ultrasound led to the diagnosis of suspected high-type anorectal malformation (A,B ...

The oxygen-evolving complex (OEC) of photosystem II contains a Mn(4)CaO(n) catalytic site, in which reactivity of bridging oxidos is fundamental to OEC function. We synthesized structurally relevant cuboidal Mn(3)MO(n) complexes (M = Mn, Ca, Sc; n = 3,4) to enable mechanistic studies of reactivity and incorporation of μ(3)-oxido moieties. We found that Mn(IV)(3)CaO(4) and Mn(IV)(3)ScO(4) were u...

PURPOSE The radiation dose rate and image quality were compared on GE OEC 9900 Elite Vas 8 and Philips Veradius fluoroscopic C-arms. METHODS Plastic (PMMA) phantoms were used to simulate various thicknesses of soft tissues encountered during fluoroscopically-guided interventional procedures while aluminum and copper phantoms were used to simulate extremities of different sizes. An image quali...

Purpose Spica casting of femoral shaft fractures is standard practice for young children. In 1983, Dr. Rang published an illustration demonstrating that radiographs can be misleading when measuring femoral fracture shortening. Today, fluoroscopy is used to determine shortening after closed reduction and spica casting. This paper aims to determine the accuracy of fluoroscopy when measuring 25 mm...

A carboxylate group of D1-Glu-189 in photosystem II has been proposed to serve as a direct ligand for the manganese cluster. Here we constructed a mutant that eliminates the carboxylate by replacing D1-Glu-189 with Gln in the cyanobacterium Synechocystis sp. PCC 6803, and we examined the resulting effects on the structural and functional properties of the oxygen-evolving complex (OEC) in photos...