Thraustochytrid protists are often abundant in coastal waters. However, their population dynamics and substrate preferences in the oceanic water column are poorly understood. We studied the abundance and distribution of thraustochytrids, bacteria and TEPs in the equatorial Indian Ocean waters during September 2003, October 2004 and September 2006. Thraustochytrids and bacteria were abundant, su...

Distribution and modulation of rabbit lymphocyte phytohaemagglutinin acceptors and concanavalin A acceptors during activation of rabbit lymphocytes have been examined by electron microscopy. Two types of cell surface acceptors have been tentatively identified, lectin binding acceptors that do not modulate, and receptors that are endocytosed when blast transformation is stimulated. All of the ce...

We have studied the effect of concanavalin A (Con A) on the rat electro-olfactogram response to several odorants. Each odorant was applied over a range of concentrations. For hydrophobic odorants whose response was affected by Con A, the diminution in response was maximal at odorant concentrations of about 1 microM in the olfactory mucus. The (odour) concentration-dependence of the change is co...

Plant lectins, because they bind to specific carbohydrate moieties, can be used to investigate the glyco-components of the cell surface. When considering the distribution, number, or mobility of lectin-binding sites, a distinction must be made between a natural distribution and a redistribution induced by binding with multivalent ligands (2, 4, 5, 8, 14, 15). In some cell types the induced redi...

Pearson, J. S. &Wood, E. J. (1974) Biochem. SOC. Trans. 2, 333-336 Poulik, M. D. (1957) Nature (London) 180, 1477-1479 VanBreernen, J. F. L.,Schuurhuis,G. J. &Van Bruggen,E. F. J. (1977) in StructureandFunction of Haemocyanin (Bannister, J. V., ed.), pp. 122-127, Springer-Verlag, Berlin and Heidelberg Weber, K. & Osborn, M. (1969) J. Biol. Chem. 244,4406-4412 Wood, E. J. (1973) Biochim. Biophys...

A gold nanoparticle-based competitive colorimetric assay uses the ensemble of Concanavalin (ConA) and mannopyranoside-encapsulated gold nanoparticles (Man-GNPs) to identify the binding partners for ConA and the binding constants are determined based on the wavelength shifts.

We describe a simplified method for purifying radiolabeled glycopeptide hormones (thyrotropin, lutropin, follitropin, and human choriogonadotropin), which results in preparations of over 95% immunoreactivity. This method is based on competitive elution from columns of the plant lectin, concanavalin A, and is applicable to glycopeptide hormones derived from several species.

In this study, we demonstrate that both highly purified T4+ and T8+ lymphocytes can produce substantial amounts of Interleukin 2(IL 2) when stimulated with the combination of concanavalin A (Con A) and phorbol myristate acetate. Furthermore, addition of IL 1 to macrophage-depleted T lymphocytes significantly increased IL 2 production by lymphocytes of either the T4+ or T8+ phenotype. These find...

The Immunogenicity of γ-irradiated tachyzoites of a highly virulent strain of Toxoplasma gondii (RH strain) was investigated. Immunized mice survived challenge infection and displayed increased interferon gamma (INF-γ ) production. On the other hand, nonimmunized controls died after 10 days and displayed a significantly decreased lymphoproliferative response to Concanavalin A (Con A). No tachyz...