With regard to the assay of heat-stable enterotoxin (ST) from Yersinia enterocolitica, we made a comparative study of the conventional infant mouse test read at 4 h and a modified infant mouse test read at 2 h. The influence of several factors, such as the medium used to prepare ST, lysing of bacterial cells from the broth cultures used to prepare ST, and the temperature at which the inoculated...

the species of lactobacillus are widely present in many natural environments that areimportant in fermented processes. among these species some belong to the lactic acid bacteria group that areuseful in fermented food. one of the species of lactobacilli group such as l. plantarum provides health benefits,but their identification is one of the main issue. in order to proceed with this project, c...

the conventional tunneling procedures for example drilling, charging, blasting, loading and supporting, are highly developed. new tunneling machines have recently come into operation and others are under construction or development. impactors are used in large numbers in the coal mining industry. face-heading machines are successfully used in new tunneling procedures in both soft and medium-har...

objective: worldwide, diabetes mellitus (dm) is an ever-increasing metabolic disorder. a promising approach to the treatment of dm is the implantation of insulin producing cells (ipc) that have been derived from various stem cells. culture conditions play a pivotal role in the quality and quantity of the differentiated cells. in this experimental study, we have applied various culture condition...

various basidiomycetes and deuteromycetes, grown in liquid and solid culture media, were compared for their laccase-producing ability and for the inducing effect of soil and agricultural residues on laccase production. laccase activity in extracts of all solid media was higher than that of the liquid media. in liquid cultures, fungal laccase activity in ex-tracts of pea (pisum sativum) straw-tr...

conventional methods of pear breeding, largely based on intra- and inter-specific hybridization, are difficult because pear is highly heterozygous, polygenic and has a long juvenile period. genetic improvements of pear cultivars are possible through induction of mutations and gene transfer by genetic engineering. a general prerequisite for these approaches is to establish an efficient plant reg...

selected genotypes of sugar beet are often maintained by conventional propagation, but much faster rates of multiplication can be now obtained through micropropagation. on the other hand, plant micropropagation using bioreactors can decrease the cost of production and makes it an economical method. in this research, the possibility of using bioreactor and optimization of the tissue culture proc...

DNA is generally considered to be chemically rather inert, but self-cleaving DNA molecules have recently been isolated from in vitro selection experiments; their properties advance the idea that, like RNA, DNA can serve as both a catalyst and an information storage medium.