نتایج جستجو برای: conventional pcr

تعداد نتایج: 428032  

Journal: :بینا 0
محمود نجابت m nejabat شیراز- خیابان پوستچی- مرکز تحقیقات چشم پزشکی پوستچی محمدرضا خلیلی mr khalili شیراز- خیابان پوستچی- مرکز تحقیقات چشم پزشکی پوستچی پریسا بدیعی p badiei شیراز- خیابان پوستچی- مرکز تحقیقات چشم پزشکی پوستچی فاطمه کشاورز فضل f keshavarz fazl شیراز- خیابان پوستچی- مرکز تحقیقات چشم پزشکی پوستچی عبدالوهاب البرزی a alborzi شیراز- خیابان پوستچی- مرکز تحقیقات چشم پزشکی پوستچی اطهر شادمانی a shadmani شیراز- خیابان پوستچی- مرکز تحقیقات چشم پزشکی پوستچی

purpose: to compare the value of conventional laboratory methods and polymerase chain reaction (pcr) in the diagnosis of fungal keratitis. methods: this cross sectional study was conducted at khalili hospital, shiraz, iran. samples were taken from thirty-eight patients with findings suspicious for fungal keratitis. corneal scrapings were used for gram, giemsa and koh stains, culture and pcr ana...

Journal: :Journal of clinical microbiology 2002
Lynne M Sloan Marlene K Hopkins P Shawn Mitchell Emily A Vetter Jon E Rosenblatt W Scott Harmsen Franklin R Cockerill Robin Patel

A rapid real-time multiplex PCR assay for detecting and differentiating Bordetella pertussis and Bordetella parapertussis in nasopharyngeal swabs was developed. This assay (LC-PCR-IS) targets the insertion sequences IS481 and IS1001 of B. pertussis and B. parapertussis, respectively, and is performed using the LightCycler (Roche Molecular Biochemicals, Indianapolis, Ind.). The analytical sensit...

2016
Simone Scherrer Daniel Frei Max Michael Wittenbrink

BACKGROUND Progressive atrophic rhinitis (PAR) in pigs is caused by toxigenic Pasteurella multocida. In Switzerland, PAR is monitored by selective culture of nasal swabs and subsequent polymerase chain reaction (PCR) screening of bacterial colonies for the P. multocida toxA gene. A panel of 203 nasal swabs from a recent PAR outbreak were used to evaluate a novel quantitative real-time PCR for t...

Journal: :Journal of clinical microbiology 2012
Maria Elena Turba Elisa Zambon Augusta Zannoni Samanta Russo Fabio Gentilini

A fundamental role for the endosymbiotic bacteria Wolbachia pipientis in the pathogenesis of Dirofilaria immitis infections has emerged in recent years. Diagnostic opportunities arising from this breakthrough have not yet been fully exploited. This study was aimed at developing conventional and real-time PCR assays to carry out a molecular survey in a convenience sample of cats living in an are...

2015
Alwin Schierenberg Martine D. Nipshagen Berna D. L. Broekhuizen Alma C. van de Pol Patricia C. J. Bruijning-Verhagen Johannes G. Kusters Rob Schuurman Sanne van Delft Marie-Josée J. Mangen Niek J. de Wit Marc J. M. Bonten

BACKGROUND Infectious intestinal disease (IID) is an important cause of morbidity in developed countries and a frequent reason for general practitioner (GP) consultation. In recent years polymerase chain reaction (PCR) based techniques have gradually replaced conventional enteropathogen detection techniques like microscopy and culture in primary care patients suspected of IID. PCR features test...

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